Ferroportin 3 is a dual‐targeted mitochondrial/chloroplast iron exporter necessary for iron homeostasis in Arabidopsis
| Autor: | Elizabeth M. Parsons, Daniel D. Chung, Fengling Hu, Claire Castellano, Avery E. Tucker, Christopher T. DaVeiga, Emily Y. Park, Jingwen Zhang, Jennifer Gallegoe Iraheta, Rong Huang, Olena K. Vatamaniuk, Ju-Chen Chia, Jeeyon Jeong, Madeline Clyne, Angie Kim, Leah J. Kim, Kaitlyn M. Tsuyuki |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Chloroplasts Iron Mutant Ferroportin Arabidopsis Plant Science Biology Mitochondrion Plant Roots 01 natural sciences Article 03 medical and health sciences Gene Expression Regulation Plant Yeasts Organelle Genetics medicine Homeostasis Amino Acid Sequence Plastid Cation Transport Proteins Conserved Sequence Arabidopsis Proteins Chemistry Wild type food and beverages Cell Biology Iron deficiency Plants Genetically Modified medicine.disease biology.organism_classification Mitochondria Cell biology Chloroplast 030104 developmental biology Mutation biology.protein 010606 plant biology & botany |
| Zdroj: | Plant J |
| ISSN: | 1365-313X 0960-7412 |
| DOI: | 10.1111/tpj.15286 |
| Popis: | Mitochondria and chloroplasts are organelles with high iron demand that are particularly susceptible to iron-induced oxidative stress. Despite the necessity of strict iron regulation in these organelles, much remains unknown about mitochondrial and chloroplast iron transport in plants. Here, we propose that Arabidopsis Ferroportin 3 (FPN3) is an iron exporter dual-targeted to mitochondria and chloroplasts.FPN3is expressed in shoots regardless of iron conditions, but its transcripts accumulate under iron deficiency in roots.fpn3mutants cannot grow as well as wild type under iron-deficient conditions and shoot iron levels are reduced infpn3mutants compared to wild type. ICP-MS measurements show that iron levels in the mitochondria and chloroplasts are increased relative to wild type, consistent with the proposed role of FPN3 as a mitochondrial/plastid iron exporter. In iron deficientfpn3mutants, abnormal mitochondrial ultrastructure was observed, whereas chloroplast ultrastructure was not affected, implying that FPN3 plays a critical role in the mitochondria. Overall, our study suggests that FPN3 is essential for optimal iron homeostasis.Significance statementIron homeostasis must be tightly controlled in the mitochondria and chloroplasts, but iron trafficking in these organelles is not fully understood. Our work suggests that FPN3 is an iron exporter required for maintaining proper iron levels in mitochondria and chloroplasts. Furthermore, FPN3 is necessary for the optimal growth and normal mitochondrial ultrastructure under iron deficiency. This study reveals the physiological role of FPN3 and advances our understanding of iron regulation in mitochondria and chloroplasts. |
| Databáze: | OpenAIRE |
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