A One-Step Homogeneous Sandwich Immunosensor for Salmonella Detection Based on Magnetic Nanoparticles (MNPs) and Quantum Dots (QDs)

Autor: Libing Wang, Hua Kuang, Chuanlai Xu, Xiujin Chen, Gang Cui, Liguang Xu, Qianqian Yong, Honghong Yin, Chifang Peng
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Salmonella
Staphylococcus aureus
Magnetic separation
quantum dots
Cross Reactions
medicine.disease_cause
Escherichia coli O157
Catalysis
Article
Inorganic Chemistry
lcsh:Chemistry
medicine
Escherichia coli
Animals
Humans
Physical and Theoretical Chemistry
magnetic separation
Magnetite Nanoparticles
Molecular Biology
fluorescent immunoassay
lcsh:QH301-705.5
Spectroscopy
Fluorescent Dyes
Immunoassay
Bacteriological Techniques
Chromatography
medicine.diagnostic_test
biology
Chemistry
Immunomagnetic Separation
Organic Chemistry
General Medicine
biology.organism_classification
equipment and supplies
Fluorescence
Molecular biology
Antibodies
Bacterial
Computer Science Applications
lcsh:Biology (General)
lcsh:QD1-999
Food Microbiology
Magnetic nanoparticles
Hazard Analysis and Critical Control Points
Bacteria
Conjugate
Zdroj: International Journal of Molecular Sciences, Vol 14, Iss 4, Pp 8603-8610 (2013)
International Journal of Molecular Sciences; Volume 14; Issue 4; Pages: 8603-8610
International Journal of Molecular Sciences
ISSN: 1422-0067
Popis: Simple immuno-magnetic separation tandem fluorescent probes based on quantum dots-antibody (QDs-Ab) were developed to detect Salmonella with sensitivity of 500 cfu mL−1. With two monoclonal antibodies, which recognize different antigenic determinant on the surface of Salmonella, we prepared antibody-coated magnetic nanoparticles (MNPs) and conjugates of QDs-Ab. The immune-magnetic beads were verified with high enrichment efficiency for Salmonella (90%). A sandwich structure formed if the Salmonella solution was mixed together with immune-beads and QDs-Ab, and the fluorescent single from QDs was related to the amount of Salmonella. A linear response between fluorescence intensity and various concentrations of Salmonella (2.5 × 103 to 1.95 × 108 cfu mL−1) were observed with this proposed method. The total assay time for Salmonella was 30 min, and no cross-reaction to other microbial strains, such as Staphylococcus aureus, Escherichia coli (E. coli) and Escherichia coli O157:H7 (E. coli O157:H7), were found using this detection system. All our results showed that the simple homogeneous immunoassay could be applied in Salmonella screening without time-consuming extra-enrichment of bacteria.
Databáze: OpenAIRE
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