Comparative analysis of secondary metabolite gene clusters in different strains of Magnaporthe oryzae
| Autor: | Meghana Shirke, Malali Gowda, Aman Nikhil, Pallabi Saha, Nazmiara Sabnam, Anantharamanan Rajamani, Atrayee Sarkar, Subhankar. Roy-Barman, H. B. Mahesh |
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| Rok vydání: | 2020 |
| Předmět: |
Magnaporthe
Secondary Metabolism Secondary metabolite Microbiology Genome Fungal Proteins 03 medical and health sciences Polyketide Ascomycota Genetics medicine Peptide Synthases Secondary metabolism Molecular Biology Gene 030304 developmental biology Plant Diseases Whole genome sequencing 0303 health sciences Alkyl and Aryl Transferases biology 030306 microbiology Strain (biology) food and beverages Oryza biology.organism_classification Multigene Family Polyketide Synthases medicine.drug |
| Zdroj: | FEMS microbiology letters. 368(1) |
| ISSN: | 1574-6968 |
| Popis: | Rice blast caused by Magnaporthe oryzae continues to be a major constraint in rice production worldwide. Rice is one of the staple crops in India and rice blast causes huge economic losses. Interestingly, the Indian subcontinent is the centre for origin and diversity of rice as well as the Magnaporthe species complex. Secondary metabolites are known to play important role in pathogenesis and M. oryzae has high potential of genes involved in secondary metabolism but, unfortunately most of them remain uncharacterized. In the present study, we analysed the draft genome assemblies of M. oryzae strains isolated from different parts of India, for putative secondary metabolite key gene (SMKG) clusters encoding polyketide synthases, non-ribosomal peptide synthetases, diterpene cyclases and dimethylallyl tryptophan synthase. Based on the complete genome sequence of 70–15 strain and its previous reports of identified SMKGs, we have identified the key genes for the interrogated strains. Expression analysis of these genes amongst different strains indicates how they have evolved depending on the host and environmental conditions. To our knowledge, this study is first of its kind where the secondary metabolism genes and their role in functional adaptation were studied across several strains of M. oryzae. |
| Databáze: | OpenAIRE |
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