Antiviral activity of NMSO3 against adenovirus in vitro
| Autor: | Shiro Shigeta, Shuichi Mori, Hisatoshi Kaneko, Keiichiro Kato |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
viruses
Biology Virus Replication medicine.disease_cause Antiviral Agents Virus Adenoviridae Cell membrane Virology Tumor Cells Cultured medicine Humans Cytotoxic T cell Cytotoxicity Pharmacology Biological activity Lipids Molecular biology N-Acetylneuraminic Acid In vitro medicine.anatomical_structure Mechanism of action Evaluation Studies as Topic Adsorption medicine.symptom |
| Zdroj: | Antiviral Research. 52:281-288 |
| ISSN: | 0166-3542 |
| Popis: | NMSO3, a sulfated sialyl lipid, was evaluated for its efficacy against adenovirus (AdV) in vitro. The median effective concentration (50% effective concentration, EC(50)) of NMSO3 against replication of AdV type 2 (AdV2), type 4 (AdV4), type 8 (AdV8) and type 37 (AdV37) was 0.21-0.71 microg/ml in HEp-2 cells and 1.01-1.41 microg/ml in MKN-28 cells. The EC(50) values of NMSO3 were lower than those of HPMPC and ddC, which were also evaluated. NMSO3 exhibited minimal cytotoxicity against HEp-2 cells and MKN-28 cells, both for which the median cytotoxic concentration (50% cytotoxic concentration, CC(50)) was more than 1000 microg/ml. NMSO3 was the most potent and selective anti-AdV compound of those examined. NMSO3 inhibited AdV infection of HEp-2 cells only when present during the virus adsorption period. A virus binding assay using radiolabeled AdV4 revealed that NMSO3 inhibited viral binding to the HEp-2 cells. NMSO3 itself bound to the virus particles, but not to the HEp-2 cell membrane. Thus, the mechanism of anti-AdV activity by NMSO3 involves inhibition of virus adsorption to cells by NMSO3 binding to viral particles. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect