3,5-Diiodo-l-Thyronine Increases Glucose Consumption in Cardiomyoblasts Without Affecting the Contractile Performance in Rat Heart
| Autor: | Riccardo Zucchi, Alessandro Saba, Sabina Frascarelli, Ginevra Sacripanti, Nhat Minh Nguyen, Sandra Ghelardoni, Leonardo Lorenzini |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Cardiac output Endocrinology Diabetes and Metabolism Glucose uptake Hemodynamics 030209 endocrinology & metabolism T2 uptake lcsh:Diseases of the endocrine glands. Clinical endocrinology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Internal medicine medicine Viability assay Original Research glucose consumption lcsh:RC648-665 T2 cardiomyoblasts Diabetes and Metabolism 030104 developmental biology chemistry Basal metabolic rate Thyronine Toxicity heart perfusion Cardiomyoblasts Glucose consumption Heart perfusion Ex vivo |
| Zdroj: | Frontiers in Endocrinology Frontiers in Endocrinology, Vol 9 (2018) |
| ISSN: | 1664-2392 |
| Popis: | 3,5-diiodo-l-thyronine (T2) is an endogenous derivative of thyroid hormone that has been suggested to regulate energy expenditure, resting metabolic rate and oxygen consumption with a mechanism that involves the activation of mitochondrial function. In this study, we focused on the cardiac effects of T2, which have been poorly investigated so far, by using both in vitro and ex vivo models. As a comparison, the response to T3 and T4 was also determined. Rat cardiomyoblasts (H9c2 cells) were used to determine T2, T3, and T4 uptake by high-performance liquid chromatography–tandem mass spectrometry. In the same experimental model, MTT test, crystal violet staining, and glucose consumption were investigated, using T2 concentrations ranging from 0.1 to 10 µM. To assess cardiac functional effects, isolated working rat hearts were perfused with T2, T3, or T4 in Krebs-Ringer buffer, and the hemodynamic variables were recorded. T2 was taken up by cardiomyoblasts, and in cell lysate T2 levels increased slowly over time, reaching higher concentrations than in the incubation medium. T2 significantly decreased MTT staining at 0.5–10 µM concentration (P |
| Databáze: | OpenAIRE |
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