Brain changes in BDNF and S100B induced by ketogenic diets in Wistar rats

Autor: Márcio Ferreira Dutra, Daniela Fraga de Souza, Maria Cristina Guerra, Adriana Fernanda Kuckartz Vizuete, Caren Luciane Bernardi, Cristiane Batassini, Ana Paula Costa, Carlos Alberto Gonçalves
Rok vydání: 2013
Předmět:
Male
medicine.medical_specialty
medicine.medical_treatment
Hippocampus
Enzyme-Linked Immunosorbent Assay
S100 Calcium Binding Protein beta Subunit
Striatum
S100B
General Biochemistry
Genetics and Molecular Biology
Pharmacology
Toxicology and Pharmaceutics(all)
Blood serum
Neurotrophic factors
Fatty Acids
Omega-6
Internal medicine
Fatty Acids
Omega-3
medicine
Animals
Nerve Growth Factors
Rats
Wistar
General Pharmacology
Toxicology and Pharmaceutics
chemistry.chemical_classification
biology
Biochemistry
Genetics and Molecular Biology(all)
Tumor Necrosis Factor-alpha
Chemistry
Brain-Derived Neurotrophic Factor
S100 Proteins
General Medicine
Ketogenic diet
Corpus Striatum
Rats
BDNF
medicine.anatomical_structure
Endocrinology
TNF-α
biology.protein
Polyunsaturated fatty acids
Diet
Ketogenic
Polyunsaturated fatty acid
Neurotrophin
Astrocyte
Zdroj: Life Sciences. 92:923-928
ISSN: 0024-3205
DOI: 10.1016/j.lfs.2013.03.004
Popis: Aims We investigated the effects of ketogenic diet (KD) on levels of tumor necrosis factor alpha (TNF-α, a classical pro-inflammatory cytokine), BDNF (brain-derived neurotrophic factor, commonly associated with synaptic plasticity), and S100B, an astrocyte neurotrophic cytokine involved in metabolism regulation. Main methods Young Wistar rats were fed during 8 weeks with control diet or two KD, containing different proportions of omega 6 and omega 3 polyunsaturated fatty acids. Contents of TNF-α, BDNF and S100B were measured by ELISA in two brain regions (hippocampus and striatum) as well as blood serum and cerebrospinal fluid. Key findings Our data suggest that KD was able to reduce the levels of BDNF in the striatum (but not in hippocampus) and S100B in the cerebrospinal fluid of rats. These alterations were not affected by the proportion of polyunsaturated fatty acids offered. No changes in S100B content were observed in serum or analyzed brain regions. Basal TNF-α content was not affected by KD. Significance These findings reinforce the importance of this diet as an inductor of alterations in the brain, and such changes might contribute to the understanding of the effects (and side effects) of KD in brain disorders.
Databáze: OpenAIRE
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