Biochemical properties of the YPT-related rab 1B protein
| Autor: | E. Vielh, Armand Tavitian, N. Touchot, Ahmed Zahraoui |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Saccharomyces cerevisiae Proteins
GTP' Genetic Vectors Molecular Sequence Data Biophysics Saccharomyces cerevisiae GTPase Biology Guanosine Diphosphate Biochemistry GTP Phosphohydrolases GTP-binding protein regulators GTP-Binding Proteins Structural Biology Mutant protein Complementary DNA Escherichia coli Genetics medicine Animals T7 RNA polymerase Amino Acid Sequence Cloning Molecular Promoter Regions Genetic Molecular Biology Binding protein Proteins DNA Cell Biology Molecular biology Recombinant Proteins Rats rab1 GTP-Binding Proteins rab GTP-Binding Proteins Mutation Guanosine Triphosphate medicine.drug |
| Zdroj: | FEBS Letters. 256:79-84 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(89)81722-3 |
| Popis: | We recently identified a novel rat cDNA: rab1B, closely related to the rab1A cDNA and to the yeast YPT1 gene. The rab1B cDNA encodes a 202 amino acid protein (22.1 kDa) that was produced in Escherichia coli under the control of the Φ 10 promoter for the T7 RNA polymerase. The rab1B protein was purified in large amounts to near homogeneity in a simplified procedure. We studied the biochemical properties of rab1B and rab1A proteins. They both bind specifically GTP and GDP and possess intrinsic GTPase activities. The rab1B Lys21 → Met mutant protein does not bind GTP, whereas the A1a65 → Thr mutant has a reduced GTPase activity and is competent for autophosphorylation in the presence of GTP.GTP binding protein; Superfamily, ras; Secretion |
| Databáze: | OpenAIRE |
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