D etection of sweet potato virus C, sweet potato virus 2 and sweet potato feathery mottle virus in Portugal
| Autor: | Carla Varanda, Maria Rosário F Félix, Maria Ivone E. Clara, M.D.M. Oliveira, Susana J Santos |
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| Jazyk: | portugalština |
| Rok vydání: | 2015 |
| Předmět: |
viruses
Molecular Sequence Data Potyvirus DNA sequencing Virus Crop Crinivirus Virology Plant virus Ipomoea batatas Phylogeny Plant Diseases Chlorosis biology Portugal digestive oral and skin physiology fungi food and beverages General Medicine Sweet potato feathery mottle virus biology.organism_classification Infectious Diseases Sweet potato GenBank Viruses |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) Agência para a Sociedade do Conhecimento (UMIC)-FCT-Sociedade da Informação instacron:RCAAP |
| Popis: | Field sweet potato plants showing virus-like symptoms, as stunting, leaf distortion, mosaic and chlorosis, were collected in southwest Portugal and tested for the presence of four potyviruses, sweet potato virus C (SPVC), sweet potato virus 2 (SPV2), sweet potato feathery mottle virus (SPFMV), sweet potato virus G (SPVG), and the crinivirus sweet potato chlorotic stunt virus (SPCSV). DsRNA fractions were extracted from symptomatic leaves and used as templates in single and multiplex RT-PCR assays using previously described specific primers for each analyzed virus. The amplified reaction products for SPVC, SPV2 and SPFMV were of expected size, and direct sequencing of PCR products revealed that they correspond to the coat protein gene (CP) and showed 98%, 99% and 99% identity, respectively, to those viruses. Comparison of the CP genomic and amino acid sequences of the Portuguese viral isolates recovered here with those of ten other sequences of isolates obtained in different countries retrieved from the GenBank showed very few differences. The application of the RT-PCR assays revealed for the first time the presence of SPVC and SPFMV in the sweet potato crop in Portugal, the absence of SPVG and SPCSV in tested plants, as well as the occurrence of triple virus infections under field conditions. |
| Databáze: | OpenAIRE |
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