Muscarinic agonists acting through M2 acetylcholine receptors stimulate the migration of an NO-sensitive guanylyl cyclase to the plasma membrane of bovine tracheal smooth muscle
| Autor: | Ramona González de Alfonzo, Marcelo J. Alfonzo, Fabiola Pláceres Uray, Itala Lippo de Becemberg |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Cytoplasm G protein Immunoblotting Receptors Cytoplasmic and Nuclear Biology Muscarinic Agonists Nitric Oxide Biochemistry Receptors G-Protein-Coupled Soluble Guanylyl Cyclase Internal medicine Muscarinic acetylcholine receptor M5 Muscarinic acetylcholine receptor Muscarinic acetylcholine receptor M4 medicine Cyclic AMP Animals Molecular Biology Acetylcholine receptor Receptor Muscarinic M2 Cell Membrane Muscarinic acetylcholine receptor M3 Muscarinic acetylcholine receptor M2 Muscle Smooth Cell Biology Muscarinic acetylcholine receptor M1 Cell biology Trachea Endocrinology Pertussis Toxin Guanylate Cyclase Cattle Subcellular Fractions |
| Zdroj: | Journal of receptor and signal transduction research. 30(1) |
| ISSN: | 1532-4281 |
| Popis: | Muscarinic agonists acting on bovine tracheal smooth muscle (BTSM) induce two separate cGMP signals, one at 20 sec associated with NO-sensitive-soluble-guanylyl-cyclase (NO-sGC) and another at 60 sec, linked to natriuretic-peptide-GC. The 20-sec-cGMP novel cascade starts with mAChRs, via unknown components, activates an NO-sGC. To unravel this cascade, in crude membranes isolated from intact BTSM strips exposed to muscarinic agonists, we detected GC activities increments at 20 sec and 60 sec. The 20-sec-GC is a NO-sensitive-GC, identified as alpha(1)beta(1)-heterodimer. In reconstitution experiments with purified plasma membranes and cytosol, muscarinic agonists induced an NO-sGC migration in a dose-dependent manner, being inhibited by muscarinic antagonists displaying an M(2)AChR profile and blocked by PTX, suggesting the involvement of G(o)/G(i) proteins. The NO-sGC related to migration was isolated and identified as an alpha(1)beta(1)-heterodimer. This work shows that muscarinic agonists in BTSM induce a massive and selective alpha(1)beta(1)-NO-sGC migration from cytoplasm to plasma membranes being responsible for the 20-sec-cGMP signal. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|