A method for measuring glycerol-blanked triglyceride concentrations by using gas chromatography-isotope dilution mass spectrometry
| Autor: | Hironori Imano, Akihiko Kitamura, Masahiko Kiyama, Isao Koyama, Hiroyasu Iso, Yoshihiro Miyamoto, Masakazu Nakamura |
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| Rok vydání: | 2020 |
| Předmět: |
Glycerol
030213 general clinical medicine Free Glycerol Chromatography Triglyceride Chemistry Glyceride Clinical Biochemistry General Medicine 030204 cardiovascular system & hematology Isotope dilution Gas Chromatography-Mass Spectrometry Hypoglycemia 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Japan Reference Values Humans Gas chromatography Serum triglycerides Triglycerides |
| Zdroj: | Annals of Clinical Biochemistry: International Journal of Laboratory Medicine. 57:253-261 |
| ISSN: | 1758-1001 0004-5632 |
| DOI: | 10.1177/0004563220921884 |
| Popis: | Background Serum triglyceride concentrations are measured as total glycerides content in many Western countries. In Japan, glycerol-blanked triglycerides (TG-GB) are measured to identify postprandial hypertriglyceridaemia and to minimize the influence of glycerol formulation on serum triglyceride values. However, TG-GB measurements have not been standardized. Therefore, we developed an efficient quantification system for total glycerides and free glycerol that allows the calculation of TG-GB concentrations. Methods We measured total glycerides and free glycerol in human serum by using gas chromatography-isotope dilution mass spectrometry and compared its performance to the reference method of the US Centers for Disease Control and Prevention (CDC). Results Our practical method of total glycerides and free glycerol quantification achieved excellent precision for both within-run and among-run coefficients of variation (Conclusions This practical method of total glycerides and free glycerol quantification enables traceability assessment of TG-GB measurements. Differences between the output values of TG-GB and the CDC reference method might result from the differences in free glycerol values. |
| Databáze: | OpenAIRE |
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