Metabolic analysis of adipose tissues in a rodent model of pre-pregnancy maternal obesity combined with offsprings on high-carbohydrate diet
| Autor: | Philip N. Baker, Xinyang Yu, Hua Zhang, Hongbo Qi, Ting-Li Han, Andi Wang, Xiaobo Zhou, Chen Zhu |
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| Rok vydání: | 2019 |
| Předmět: |
Blood Glucose
Male 0301 basic medicine medicine.medical_specialty Offspring Adipocytes White Adipose tissue White adipose tissue Biology Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Pregnancy Internal medicine Brown adipose tissue Dietary Carbohydrates medicine Animals Weaning Obesity Mice Knockout chemistry.chemical_classification Fatty acid metabolism Body Weight Fatty acid Cell Biology medicine.disease Mice Inbred C57BL Pregnancy Complications Adipocytes Brown 030104 developmental biology Endocrinology medicine.anatomical_structure Adipose Tissue chemistry 030220 oncology & carcinogenesis Metabolome Receptors Leptin Female Diet Carbohydrate Loading |
| Zdroj: | Experimental Cell Research. 381:29-38 |
| ISSN: | 0014-4827 |
| Popis: | Maternal obesity is associated with adverse effects on the health of offsprings. Consumption of a high-carbohydrate (HC) diet has been found to promote abnormal fatty acid metabolism in adipose tissue. Therefore, we hypothesised that maternal obesity combined with an offspring HC diet would alter the fatty acid metabolism of adipose tissue and subsequently contribute to offspring obesity. Leprdb/+ mice were used to model pre-pregnancy maternal obesity and the C57BL/6 wildtype were used as a control group. Offspring were fed either HC diet or a normal-carbohydrate (NC) diet after weaning. Brown adipose tissue (BAT) and white adipose tissue (WAT) were collected from offspring at 20 weeks of age and their fatty acid metabolome was characterized using gas chromatography-mass spectrometry. We found that HC diet increased the body weight of offspring (males increased by 14.70% and females increased by 1.05%) compared to control mothers. However, maternal obesity alone caused a 7.9% body weight increase in female offspring. Maternal obesity combined with an offspring HC diet resulted in dynamic alterations of the fatty acid profiles of adipose tissue in male offspring. Under the impact of a HC diet, the fatty acid metabolome was solely elevated in female WAT, whereas, the fatty acid metabolites in BAT showed a similar trend in the male and female offsprings. 6,9-octadecadienoic acid and 12,15-cis-octadecatrienoic acid were significantly affected in female WAT, in response to offspring consumption of a HC diet. Our study demonstrated that maternal obesity and offspring HC diet have different metabolic effects on adipose tissue in male and female offsprings. |
| Databáze: | OpenAIRE |
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