Anti-oxidative Activity of Hydrolysate from Rice Bran Protein in HepG2 Cells
Autor: | Kayoko Kawakami, Tadashi Hatanaka, Seiji Tsuboi, Akiko Fujita, Chie Moritani, Koji Kawakami |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Cell Survival NF-E2-Related Factor 2 Protein Hydrolysates Pharmaceutical Science Tripeptide medicine.disease_cause Antioxidants Hydrolysate 03 medical and health sciences chemistry.chemical_compound medicine Humans Cytotoxicity Transcription factor Plant Proteins Pharmacology chemistry.chemical_classification Ethanol Dose-Response Relationship Drug Chemistry Oryza Hep G2 Cells General Medicine Glutathione Oxidative Stress 030104 developmental biology Enzyme Biochemistry Oxidative stress Signal Transduction |
Zdroj: | Biological & Pharmaceutical Bulletin. 40:984-991 |
ISSN: | 1347-5215 0918-6158 |
Popis: | Glutathione (GSH) is an ubiquitous thiol-containing tripeptide, which plays important roles in cellular protection from oxidative stress. In our search for a dietary source that can increase GSH levels, we discovered that a 24 h treatment of HepG2 cells with rice bran protein hydrolysate (RBPH), prepared by Umamizyme G-catalyzed hydrolysis, increased the GSH content in a dose-dependent manner. RBPH elevated the expression levels of γ-glutamylcysteine synthetase (γ-GCS), which constitutes the rate-limiting enzyme of GSH synthesis, and of another two enzymes, hemeoxygenase-1 (HO-1) and reduced nicotinamide adenine dinucleotide (phosphate): quinone oxidoreductase 1 (NQO1). This induction was preceded by the accumulation of nuclear factor erythroid 2-related factor 2 (Nrf2) inside the nucleus, which is a key transcription factor for the expression of the γ-GCS, HO-1, and NQO1. Pre-treatment of cells with RBPH produced a significant protective effect against cytotoxicity caused by H2O2 or ethanol. These results indicate that RBPH exerts a protective effect against oxidative stress by modulating GSH levels and anti-oxidative enzyme expression via the Nrf2 pathway. |
Databáze: | OpenAIRE |
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