Role of regulatory T cells in coronavirus-induced acute encephalitis
| Autor: | Jingxian Zhao, Daniela Anghelina, Kathryn Trandem, Stanley Perlman |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Rodent model
Chemokine Adoptive cell transfer Coronaviridae Infections medicine.medical_treatment medicine.disease_cause T-Lymphocytes Regulatory Epitope Virus Article Mice Mouse hepatitis virus Effector CD4 T cells T-Lymphocyte Subsets Virology medicine Animals Encephalitis Viral Coronavirus Mice Inbred BALB C biology Brain Regulatory T cells Fibroblasts Acute encephalitis medicine.disease biology.organism_classification Survival Analysis Disease Models Animal Cytokine Immunology biology.protein Encephalitis |
| Zdroj: | Virology |
| ISSN: | 1096-0341 |
| Popis: | C57BL/6 mice infected with mouse hepatitis virus, strain JHM (JHMV) develop a rapidly fatal acute encephalitis. Previously, we showed that this disease is partially CD4 T cell-mediated since infection with a recombinant JHMV (rJ) mutated in only a single immunodominant CD4 T cell epitope (epitope M133, rJ.MY135Q) results in a nonlethal disease. Increased mortality correlated with a greater number of JHMV-specific CD4 T cells in the brains of rJ compared to rJ.MY135Q-infected mice. Here, we extend these results to show that the diminished number of virus-specific T cells correlates with a reduced cytokine/chemokine response in the infected brain. We also show that regulatory CD4 T cells (Tregs) are critical for mild disease in rJ.MY135Q-infected mice because their depletion results in increased mortality. Further, a relative paucity of Tregs characterizes lethal infection because adoptive transfer of Tregs into rJ-infected mice increases survival from 0% to 50%. These results support the notion that clinical disease in coronavirus-induced acute encephalitis results from a balance between factors critical for virus clearance, such as virus-specific effector T cells and anti-inflammatory elements, such as Tregs. These findings also show that unlike chronic infections, in which an excessive number of Tregs contributes to pathogen persistence, Tregs in the setting of acute encephalitis may help to limit immunopathological disease without delaying virus clearance. |
| Databáze: | OpenAIRE |
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