Hepatoprotective mechanism of freshwater clam extract alleviates non-alcoholic fatty liver disease: elucidatedin vitroandin vivomodels

Autor: Chun-Ju Chang, Yeuk-Chuen Liu, Jing-Jen Lin, Bonnie Sun Pan, Mong-Fong Lee, Min-Hsiung Pan
Rok vydání: 2018
Předmět:
Male
0301 basic medicine
medicine.medical_specialty
Meat
food.ingredient
Aspartate transaminase
Protective Agents
digestive system
Mice
03 medical and health sciences
0302 clinical medicine
food
Non-alcoholic Fatty Liver Disease
Internal medicine
medicine
Animals
Humans
Carnitine
Triglycerides
chemistry.chemical_classification
Carnitine O-Palmitoyltransferase
biology
Chemistry
Fatty Acids
Fatty liver
nutritional and metabolic diseases
Tilapia
General Medicine
medicine.disease
Bivalvia
Mice
Inbred C57BL
Fatty acid synthase
Cholesterol
030104 developmental biology
Endocrinology
Liver
Alanine transaminase
Lipogenesis
biology.protein
lipids (amino acids
peptides
and proteins)
030211 gastroenterology & hepatology
Acyl-CoA Oxidase
Fatty Acid Synthases
Food Science
medicine.drug
Polyunsaturated fatty acid
Zdroj: Food & Function. 9:6315-6325
ISSN: 2042-650X
2042-6496
Popis: Freshwater clams (Corbicula fluminea) have long been used as a folk remedy in Chinese tradition. Their hot-water extract has been commercialized as a functional drink for liver protection. The objective of this study was to develop a product of the residual clam meat (FCR) and assess its functional compounds. The ethanol extract of FCR, designated FCRE, was identified to comprise phytosterols, polyunsaturated fatty acids (PUFAs) and carotenoids. FCRE significantly reduced lipid accumulation and cell death in HepG2 cells via decreased fatty acid synthase (FAS) activity and increased activities of carnitine palmitoyltransferase (CPT) and acyl-CoA oxidase (ACO), indicative of suppressed lipogenesis and increased β-oxidation of fatty acids. In tilapia fed with high-fat diet (HFD), FCRE mitigated nonalcoholic steatohepatitis (NASH), which was evidenced by decreased levels of plasma aspartate transaminase (AST) and alanine transaminase (ALT), in addition to reduced total cholesterol and accumulation of triacylglycerols, particularly those of saturated and monounsaturated fatty acids. FCRE also suppressed stearoyl-CoA desaturase-1 (SCD-1) index, increased the PUFAs' n3/n6 ratio, and reduced prostaglandin E2 (PGE2) and inflammatory infiltrates in tilapia liver. Tilapia fed with HFD for 2 weeks displayed NASH symptoms, while mice took 10 weeks to display NASH symptoms. No previous study has been reported on the potential use of tilapia as an NASH model for pre-screening hepatoprotective-functional foods.
Databáze: OpenAIRE
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