Essential structures of a self-aminoacylating RNA
| Autor: | Michael Yarus, Mali Illangasekare, Oleh Kovalchuke |
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| Rok vydání: | 1997 |
| Předmět: |
Models
Molecular Adenosine monophosphate Stereochemistry Acylation Phenylalanine Molecular Sequence Data Aminoacylation Active center chemistry.chemical_compound Structural Biology RNA Catalytic Nucleotide Binding site Molecular Biology Sequence Deletion chemistry.chemical_classification Binding Sites Base Sequence biology Ribozyme RNA Adenosine Monophosphate chemistry Metals Helix biology.protein Nucleic Acid Conformation |
| Zdroj: | Journal of Molecular Biology. 274:519-529 |
| ISSN: | 0022-2836 |
| DOI: | 10.1006/jmbi.1997.1414 |
| Popis: | Comparison of six independent self-aminoacylating RNAs derived from selection-amplification, as well as deletion, addition, substitution, fragmentation of one particular RNA, are used to analyze the requirements for the RNA-catalyzed aminoacylation. All elements required for catalysis by one RNA family: sequence at the 3' acceptor end, calcium and magnesium sites, as well as the Phe-AMP substrate site and the essential 5' triphosphate terminus, are closely grouped near a bihelix junction in the parental molecule. All elements of the active center for aminoacyl transfer can therefore be captured by a peripherally-deleted helix junction RNA, defining a much smaller 43 nucleotide ribozyme, of which only 17 nucleotides were initially randomized. It appears that a complex RNA active center can be assembled by specifying unexpectedly few nucleotides, perhaps with a critical contribution from an essential calcium ion. |
| Databáze: | OpenAIRE |
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