Adhesion of Pseudomonas aeruginosa to contact lenses after exposure to multi-purpose lens care solutions
| Autor: | H. C. van der Mei, Jacob de Vries, Henk J. Busscher, GM Bruinsma |
|---|---|
| Přispěvatelé: | Man, Biomaterials and Microbes (MBM), Personalized Healthcare Technology (PHT) |
| Rok vydání: | 2001 |
| Předmět: |
X-ray photoelectron spectroscopy
Materials science Polymacon Parallel-plate flow chamber lens care solution chemistry.chemical_element Oxygen law.invention ADHERENCE Optics law hydrogel contact lenses Materials Chemistry MICROORGANISMS integumentary system business.industry Biomaterial Surfaces and Interfaces General Chemistry Adhesion bacterial adhesion EFFICACY Surfaces Coatings and Films Lens (optics) Contact lens MICROBIAL KERATITIS chemistry Chemical engineering Mechanics of Materials Pseudomonas aeruginosa business |
| Zdroj: | Journal of Adhesion Science and Technology, 15(12), 1453-1461. TAYLOR & FRANCIS LTD |
| ISSN: | 1568-5616 0169-4243 |
| DOI: | 10.1163/156856101753213303 |
| Popis: | Elemental surface compositions of contact lenses were measured after exposure to different lens care solutions (LCS) using X-ray photoelectron spectroscopy and were related to adhesion and detachment of Pseudomonas aeruginosa. Etafilcon A and polymacon contact lenses, prior to and after exposure to LCS were fixed on the bottom plate of a parallel plate flow chamber after which P. aeruginosa #3 was allowed to adhere for 2 h. After adhesion, bacterial detachment was stimulated by perfusing the chamber with an LCS or by passing an air-bubble through the chamber. After exposure to an LCS, the adhesion of P. aeruginosa #3 could either be enhanced or decreased, depending on the contact lens and LCS involved. Initial deposition rates of P aeruginosa #3 could not be related with changes in elemental surface composition of the contact lenses, but decreased with an increasing ratio of oxygen involved in O=C bonds relative to oxygen in O-C bonds. P. aeruginosa #3 adhered tenaciously to both types of contact lenses and the passage of an air-bubble through the flow chamber detached only up to 9% of the adhering bacteria. Alternatively, the LCS most effective in decreasing bacterial adhesion after exposure (LCS A), was least effective in detaching adhering R aeruginosa #3 (8-15%), while the other LCS detached up to 42% of adhering bacteria. In conclusion, different LCS have different abilities to detach the adhering P aeruginosa #3 from contact lens surfaces and all leave adsorbed components on the surface after soaking. Adsorbed components rich in O-C bonds increased adhesion of P. aeruginosa #3 under the conditions used in this study and should, therefore, be avoided. |
| Databáze: | OpenAIRE |
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