Differential expression and immunolocalization of antioxidant enzymes in Entamoeba histolytica isolates during metronidazole stress
| Autor: | Lakshmi Rani Iyer, Anil Kumar Verma, Jaishree Paul, Nishant Singh |
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| Rok vydání: | 2014 |
| Předmět: |
Article Subject
Population lcsh:Medicine India Biology General Biochemistry Genetics and Molecular Biology Antioxidants Gene Expression Regulation Enzymologic Microbiology Entamoeba histolytica Stress Physiological Metronidazole parasitic diseases medicine Humans Amoebiasis RNA Messenger education chemistry.chemical_classification Cell Nucleus education.field_of_study General Immunology and Microbiology lcsh:R Entamoeba General Medicine Amebiasis Peroxiredoxins respiratory system biology.organism_classification medicine.disease Protein subcellular localization prediction Enzyme chemistry Peroxiredoxin medicine.drug Research Article |
| Zdroj: | BioMed Research International BioMed Research International, Vol 2014 (2014) |
| ISSN: | 2314-6141 |
| Popis: | Entamoeba histolyticainfections are endemic in the Indian subcontinent. Five to eight percent of urban population residing under poor sanitary conditions suffers fromEntamoebainfections. Metronidazole is the most widely prescribed drug used for amoebiasis. In order to understand the impact of metronidazole stress on the parasite, we evaluated the expression of two antioxidant enzymes, peroxiredoxin and FeSOD, inEntamoeba histolyticaisolates during metronidazole stress. The results reveal that, under metronidazole stress, the mRNA expression levels of these enzymes did not undergo any significant change. Interestingly, immunolocalization studies with antibodies targeting peroxiredoxin indicate differential localization of the protein in the cell during metronidazole stress. In normal conditions, all theEntamoebaisolates exhibit presence of peroxiredoxin in the nucleus as well as in the membrane; however with metronidazole stress the protein localized mostly to the membrane. The change in the localization pattern was more pronounced when the cells were subjected to short term metronidazole stress compared to cells adapted to metronidazole. The protein localization to the cell membrane could be the stress response mechanism in these isolates. Colocalization pattern of peroxiredoxin with CaBp1, a cytosolic protein, revealed that the membrane and nuclear localization was specific to peroxiredoxin during metronidazole stress. |
| Databáze: | OpenAIRE |
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