Increased resistin suggests poor prognosis and promotes development of lung adenocarcinoma

Autor: Jing Chen, Cui‑Cui Zhao, Chuan‑Gui Zhang, Yan Liu, Rui‑Fang Niu
Rok vydání: 2018
Předmět:
0301 basic medicine
Male
Cancer Research
endocrine system diseases
Mice
0302 clinical medicine
Nude mouse
Cell Movement
Resistin
biology
General Medicine
Articles
respiratory system
Middle Aged
Prognosis
Tumor Burden
Up-Regulation
Gene Expression Regulation
Neoplastic
Oncology
030220 oncology & carcinogenesis
Adenocarcinoma
Immunohistochemistry
Female
hormones
hormone substitutes
and hormone antagonists
cancer tissue
Adult
biological behavior
Adenocarcinoma of Lung
03 medical and health sciences
Cell Line
Tumor
medicine
Animals
Humans
Lung cancer
Aged
Cell Proliferation
Neoplasm Staging
Oncogene
business.industry
Cancer
nutritional and metabolic diseases
medicine.disease
biology.organism_classification
lung adenocarcinoma
Molecular medicine
Survival Analysis
030104 developmental biology
A549 Cells
Cancer research
business
Neoplasm Transplantation
Zdroj: Oncology Reports
ISSN: 1791-2431
Popis: Resistin is considered to be a risk factor for several types of cancer, but its functions are controversial and not well studied in lung cancer. The present study is aimed to investigate the expression of resistin in lung adenocarcinoma tissues, in order to evaluate its association with the clinicopathological characteristics of cancer patients and to investigate the effects of resistin in lung adenocarcinoma cells. A total of 70 pairs of lung adenocarcinoma tissues and normal tissues were collected and immunohistochemistry was performed to examine resistin expression. Resistin overexpressed cells were established by plasmid transfection in A549 or H1975 cells. Alterations in cell proliferation, apoptosis, migration and invasion were analyzed in vitro. A nude mouse tumorigenicity assay was used to test the effect of resistin in vivo. High expression of resistin was predominantly observed in lung adenocarcinoma tissues but not in adjacent normal lung tissues. Resistin expression was significantly associated with increased tumor size, clinical stage as well as lymph node metastasis while negatively associated with progression-free survival (PFS) and overall survival (OS). Expression of resistin was an independent risk factor for PFS and OS. Overexpression of resistin promoted significant proliferation, migration and invasion, while also inhibited apoptosis in vitro. Resistin also promoted tumor formation in nude mice. The potential molecular mechanism was also investigated by in vitro experiments. In conclusion, the present study revealed that a high level of resistin expression in lung adenocarcinoma tissues is associated with poor clinicopathological status and survival. Resistin, which promotes the development of lung adenocarcinoma in vitro and in vivo may be a novel target for lung adenocarcinoma.
Databáze: OpenAIRE
Externí odkaz: