Dual-Drug-Loaded Silk Fibroin/PLGA Scaffolds for Potential Bone Regeneration Applications
| Autor: | Wendi Ma, Dahui Sun, Yilong Wang, Wenying Dong, Mei Zhang, Jihang Yao |
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| Rok vydání: | 2019 |
| Předmět: |
Scaffold
Materials science Article Subject Biocompatibility Fibroin 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Bone morphogenetic protein 01 natural sciences 0104 chemical sciences PLGA chemistry.chemical_compound chemistry Tissue engineering Nanofiber lcsh:Technology (General) Biophysics lcsh:T1-995 General Materials Science 0210 nano-technology Bone regeneration |
| Zdroj: | Journal of Nanomaterials, Vol 2019 (2019) |
| ISSN: | 1687-4129 1687-4110 |
| Popis: | Developing scaffold materials with excellent biocompatibility, mechanical properties, and controlled drug release properties is vital to tissue engineering. In this study, we fabricated silk fibroin (SF)/poly(lactide-co-glycolide) (PLGA) nanofiber scaffolds containing recombinant human bone morphogenetic protein 2 (rhBMP2) and dexamethasone (DXM) via coaxial electrospinning, which were used in in vitro bone formation with rat bone marrow mesenchymal stem cells (rBMSCs). An in vitro drug release study was adopted to evaluate the sustained release potential of the core-shell structured nanofibers. Furthermore, we detected the potential of the SF/PLGA nanofiber membrane in vitro. In vitro studies showed that rhBMP2 still remained active on the nanofiber membrane. In addition, the dual-drug-loaded nanofiber membrane showed an early burst release of DXM and late sustained release of rhBMP2. rhBMP2 and DXM exhibited strong osteogenic differentiation potential when they acted on rBMSCs. Therefore, the SF/PLGA nanofiber membrane loaded with rhBMP2 and DXM has great potential for the enhancement of bone regeneration. |
| Databáze: | OpenAIRE |
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