Identification of genes associated with tumorigenesis of meibomian cell carcinoma by microarray analysis
| Autor: | Arun Kumar, Venkatesh C. Prabhakaran, Syril Dorairaj, D. Ravi Prakash, Sanjukta Chakraborty |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
Microarray Microarrays Biology medicine.disease_cause Eyelid Neoplasms Expression profile Meibomian cell carcinoma Gene expression medicine JAK/STAT pathway Genetics Humans RNA Messenger Gene Aged Oligonucleotide Array Sequence Analysis Microarray analysis techniques Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Cancer JAK-STAT signaling pathway food and beverages Meibomian Glands Middle Aged medicine.disease Neoplasm Proteins Gene Expression Regulation Neoplastic Case-Control Studies Cancer research MAPK signaling pathway DNA microarray Carcinogenesis Signal Transduction |
| Zdroj: | Genomics. 90(5) |
| ISSN: | 0888-7543 |
| Popis: | Meibomian cell carcinoma (MCC) is a malignant tumor of the meibomian glands located in the eyelids. No information exists on the cytogenetic and genetic aspects of MCC. There is no report on the gene expression profile of MCC. Thus there is a need, for both scientific and clinical reasons, to identify genes and pathways that are involved in the development and progression of MCC. We analyzed the gene expression profile of MCC by the microarray technique. Forty-four genes were upregulated and 149 genes were downregulated in MCC. Differential expression data were confirmed for 5 genes by semiquantitative RT-PCR in MCC tumors: GTF2H4, RBM12, UBE2D3, DDX17, and LZTS1. We found dysregulation of two major pathways in MCC: MAPK and JAK/STAT. Clusters of genes on chromosomes 1, 12, and 19 were dysregulated in MCC. The data presented here will facilitate the identification of specific markers and therapeutic targets for the treatment of MCC patients. |
| Databáze: | OpenAIRE |
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