Role of the C-terminal region of dextransucrase from Leuconostoc mesenteroides IBT-PQ in cell anchoring
| Autor: | Luis M. Ledezma-Candanoza, Clarita Olvera, Agustín López-Munguía, José Luis Fernández-Vázquez |
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| Rok vydání: | 2007 |
| Předmět: |
Models
Molecular chemistry.chemical_classification biology Sequence analysis Molecular Sequence Data biology.organism_classification medicine.disease_cause Microbiology Molecular biology Recombinant Proteins Dextransucrase Cell wall Enzyme Biochemistry chemistry Cell Wall Glucosyltransferases Leuconostoc mesenteroides medicine Amino Acid Sequence Cloning Molecular Gene Escherichia coli Leuconostoc Binding domain |
| Zdroj: | Microbiology. 153:3994-4002 |
| ISSN: | 1465-2080 1350-0872 |
| DOI: | 10.1099/mic.0.2007/008854-0 |
| Popis: | dsrP, a gene that encodes a cell-associated dextransucrase produced by Leuconostoc mesenteroides IBT-PQ, was isolated, sequenced and expressed in Escherichia coli. From sequence analysis, seven repeat units in the N-terminal region were found, as well as five cell wall binding repeats in the C-terminal region. A model of the C-terminal domain of dextransucrase was built based on the solenoid structure of the cell wall binding domain already described in LytA. By experiments involving direct interactions of the enzyme with L. mesenteroides cells, as well as among the cells and the single C-terminal domain expressed in E. coli, evidence was obtained concerning the anchoring function of this region in cell-associated dextransucrase, a function which may be independent of its capacity to bind dextran. |
| Databáze: | OpenAIRE |
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