Evaluation of toxicity and degradation of a chlorophenol mixture by the laccase produced by Trametes pubescens
| Autor: | Carlos J. Alméciga-Díaz, Juan C. González, Angela Espejo, Alexander Rodríguez, Sandra Constanza Medina, V. Sarria, Ingrid J. Gaitan, Oscar F. Sánchez, Johann F. Osma |
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| Rok vydání: | 2011 |
| Předmět: |
Trametes
Chlorophenol chemistry.chemical_classification Laccase Environmental Engineering Chromatography biology Molecular mass Cell Survival Renewable Energy Sustainability and the Environment Trametes pubescens Bioengineering General Medicine Biodegradation biology.organism_classification Pentachlorophenol chemistry.chemical_compound Biodegradation Environmental Enzyme chemistry Toxicity Waste Management and Disposal Chlorophenols |
| Zdroj: | Bioresource Technology. 102:3632-3635 |
| ISSN: | 0960-8524 |
| DOI: | 10.1016/j.biortech.2010.11.040 |
| Popis: | In this study, the biodegradation of a mixture of 2-chlorophenol (2-CP), 2,4-dichlorophenol (2,4-DCP), 2,4,6-trichlorophenol (2,4,6-TCP) and pentachlorophenol (PCP) using the laccase produced by the white-rot fungus Trametes pubescens CBS 696.94 was evaluated. Two laccase isoenzymes with molecular weights of about 60 and 120 kDa were identified in the enzymatic crude extract. The highest laccase activity with syringaldazine was observed with pH 6.0 and 60 °C, while with 2,2-azino-bis(3-ethylbenzothiazoline-6) sulphonic acid the highest activity was observed between 50 and 60 °C and 3.0–4.0 pH. A biodegradation of 100%, 99%, 82.1% and 41.1% for 2-CP, 2,4-DCP, 2,4,6-TCP and PCP, respectively, was observed after 4 h of reaction. The reduction in chlorophenols concentration allowed 90% reduction in mixture toxicity. In summary, these results show the feasibility of a laccase enzymatic crude extract from T. pubescens for the reduction of concentration and toxicity of chlorophenols. |
| Databáze: | OpenAIRE |
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