Angiotensin II type 1 receptor: role in renal growth and gene expression during normal development
| Autor: | H. Dagli, K. M. Geary, Allen D. Everett, Robert L. Chevalier, A. Tufro-McReddie, R. A. Gomez, Robert M. Carey, D W Johns |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Male
Aging medicine.medical_specialty Physiology Gene Expression Tetrazoles Blood Pressure In situ hybridization Biology Kidney Losartan Rats Sprague-Dawley Reference Values Internal medicine Renin Renin–angiotensin system Gene expression medicine Animals RNA Messenger Receptors Angiotensin Angiotensin II receptor type 1 Biphenyl Compounds Imidazoles Angiotensin II Rats Biphenyl compound Endocrinology medicine.anatomical_structure Animals Newborn cardiovascular system hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | American Journal of Physiology-Renal Physiology. 266:F911-F918 |
| ISSN: | 1522-1466 1931-857X |
| DOI: | 10.1152/ajprenal.1994.266.6.f911 |
| Popis: | To determine whether angiotensin II (ANG II) modulates renal growth and renin and angiotensin type 1 (AT1) gene expression via AT1 during development, weanling rats were given ANG II antagonist losartan (DuP 753) for 3 wk. Body weight (g), kidney weight (g), and kidney weight-to-body weight ratio were lower in losartan-treated rats (162 +/- 7, 1.6 +/- 0.06, and 9.5 +/- 0.1 x 10(-3)) than in control rats (184 +/- 5, 1.8 +/- 0.07, and 10.1 +/- 0.1 x 10(-3); P < 0.05). Renal DNA content (mg/kidney) was lower in losartan-treated (2.4 +/- 0.17) than in control rats (3.3 +/- 0.31; P < 0.05), whereas protein-to-DNA and RNA-to-DNA ratios were similar in losartan-treated and control rats. Renin mRNA levels were sevenfold higher in losartan-treated than in control rats, as determined by quantitative standardized dot blot analysis. In addition, blockade of AT1 with losartan induced recruitment of renin-synthesizing and renin-containing cells in the renal vasculature, as determined by immunocytochemistry and in situ hybridization. To establish whether AT1 blockade has a direct effect on renin gene expression, freshly isolated renin-producing cells were exposed in vitro to losartan (10(-6) M) or culture media (control). Losartan induced a twofold increase in steady-state renin mRNA levels above control (P < 0.05). Intrarenal AT1 mRNA levels were not altered by losartan given either in vivo or in vitro to freshly dispersed cells. To define whether immature renin-secreting cells are responsive to ANG II, renin release was determined by reverse hemolytic plaque assay.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|