Increased concentrations of endogenous 13-cis- and all-trans-retinoic acids in diffuse idiopathic skeletal hyperostosis, as demonstrated by HPLC
| Autor: | G. Lecomte, Jésus Garcia, Mazières B, J.P. Thouvenot, A.P. De Leenheer, Willy E. Lambert, J. Arlet, B. Periquet |
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| Rok vydání: | 1991 |
| Předmět: |
Adult
Male Hyperostosis Clinical Biochemistry Retinoic acid Tretinoin Endogeny Biochemistry High-performance liquid chromatography chemistry.chemical_compound medicine Humans Isotretinoin Vitamin A Chromatography High Pressure Liquid Aged Diffuse Idiopathic Skeletal Hyperostosis Aged 80 and over Hyperostosis Diffuse Idiopathic Skeletal Chromatography Biochemistry (medical) Retinol All trans General Medicine Middle Aged medicine.disease Retinol-Binding Proteins chemistry Female Spectrophotometry Ultraviolet Quantitative analysis (chemistry) |
| Zdroj: | Clinica Chimica Acta. 203:57-65 |
| ISSN: | 0009-8981 |
| DOI: | 10.1016/0009-8981(91)90156-7 |
| Popis: | Endogenous 13- cis - and all- trans -retinoic acids have been quantitated in human serum using a solvent extraction procedure followed by isocratic reversed phase high performance liquid chromatography and UV detection. In healthy adults, after an overnight fasting period, the concentrations of 13- cis - and all- trans -retinoic acids yielded 5.3 ± 2.43 nmol/l and 11.8 ± 3.3 nmol/l, respectively (mean ±SD). The method has been successfully applied to the analysis of both isomers in serum from patients with idiopathic skeletal hyperostosis in whom, the 13- cis - as well as all- trans -retinoic acid levels were raised as compared to the control group. |
| Databáze: | OpenAIRE |
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