CD11c identifies microbiota and EGR2-dependent MHCII+ serous cavity macrophages with sexually dimorphic fate in mice

Autor: Calum C. Bain, Pieter A. Louwe, Nicholas J. Steers, Alberto Bravo‐Blas, Lizi M. Hegarty, Clare Pridans, Simon W.F. Milling, Andrew S. MacDonald, Dominik Rückerl, Stephen J. Jenkins
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Bain, C C, Louwe, P A, Steers, N, Bravo-Blas, A, Hegarty, L, Pridans, C, Milling, S, MacDonald, A S, Ruckerl, D & Jenkins, S J 2022, ' CD11c identifies microbiota and EGR2-dependent MHCII+ serous cavity macrophages with sexually dimorphic fate in mice ', European Journal of Immunology, vol. 52, no. 8, pp. 1243-1257 . https://doi.org/10.1002/eji.202149756
Bain, C C, Louwe, P A, Steers, N J, Bravo-Blas, A, Hegarty, L M, Pridans, C, Milling, S W F, MacDonald, A S, Rückerl, D & Jenkins, S J 2022, ' CD11c identifies microbiota and EGR2-dependent MHCII+ serous cavity macrophages with sexually dimorphic fate in mice ', European journal of immunology . https://doi.org/10.1002/eji.202149756
ISSN: 0014-2980
DOI: 10.1002/eji.202149756
Popis: The murine serous cavities contain a rare and enigmatic population of short-lived F4/80 loMHCII + macrophages but what regulates their development, survival, and fate is unclear. Here, we show that mature F4/80 loMHCII + peritoneal macrophages arise after birth, but that this occurs largely independently of colonization by microbiota. Rather, microbiota specifically regulate development of a subpopulation of CD11c + cells that express the immunoregulatory cytokine RELM-α, are reliant on the transcription factor EGR2, and develop independently of the growth factor CSF1. Furthermore, we demonstrate that intrinsic expression of RELM-α, a signature marker shared by CD11c + and CD11c – F4/80 loMHCII + cavity macrophages, regulates survival and differentiation of these cells in the peritoneal cavity in a sex-specific manner. Thus, we identify a previously unappreciated diversity in serous cavity F4/80 loMHCII + macrophages that is regulated by microbiota, and describe a novel sex and site-specific function for RELM-α in regulating macrophage endurance that reveals the unique survival challenge presented to monocyte-derived macrophages by the female peritoneal environment.
Databáze: OpenAIRE
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