Adult brain neurons require continual expression of the schizophrenia-risk gene Tcf4 for structural and functional integrity
| Autor: | Deepanjali Dwivedi, Mohammad Shariq, Dipannita Sarkar, Upinder S. Bhalla, Ronald Naumann, Nirmal Krishnan, Hiyaa S. Ghosh |
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| Rok vydání: | 2021 |
| Předmět: |
Neurons
Gene knockdown Gene regulatory network Brain Neurosciences. Biological psychiatry. Neuropsychiatry Context (language use) Haploinsufficiency TCF4 Biology Molecular neuroscience Embryonic stem cell Article Transcriptome Disease Models Animal Mice Cellular and Molecular Neuroscience Psychiatry and Mental health Schizophrenia Animals Neuroscience Gene Biological Psychiatry RC321-571 |
| Zdroj: | Translational Psychiatry, Vol 11, Iss 1, Pp 1-11 (2021) Translational Psychiatry |
| ISSN: | 2158-3188 |
| DOI: | 10.1038/s41398-021-01618-x |
| Popis: | The schizophrenia-risk gene Tcf4 has been widely studied in the context of brain development using mouse models of haploinsufficiency, in utero knockdown and embryonic deletion. However, Tcf4 continues to be abundantly expressed in adult brain neurons where its functions remain unknown. Given the importance of Tcf4 in psychiatric diseases, we investigated its role in adult neurons using cell-specific deletion and genetic tracing in adult animals. Acute loss of Tcf4 in adult excitatory neurons in vivo caused hyperexcitability and increased dendritic complexity of neurons, effects that were distinct from previously observed effects in embryonic-deficiency models. Interestingly, transcriptomic analysis of genetically traced adult-deleted FACS-sorted Tcf4-knockout neurons revealed that Tcf4 targets in adult neurons are distinct from those in the embryonic brain. Meta-analysis of the adult-deleted neuronal transcriptome from our study with the existing datasets of embryonic Tcf4 deficiencies revealed plasma membrane and ciliary genes to underlie Tcf4-mediated structure-function regulation specifically in adult neurons. The profound changes both in the structure and excitability of adult neurons upon acute loss of Tcf4 indicates that proactive regulation of membrane-related processes underlies the functional and structural integrity of adult neurons. These findings not only provide insights for the functional relevance of continual expression of a psychiatric disease-risk gene in the adult brain but also identify previously unappreciated gene networks underpinning mature neuronal regulation during the adult lifespan. |
| Databáze: | OpenAIRE |
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