Inhibition of γδ-TcR or IL17a Reduces T-Cell and Neutrophil Infiltration after Ischemia/Reperfusion Injury in Mouse Liver

Autor: Saant Al Mogrampi, Christina Boumpoureka, Hara Afaloniati, Maria Lagou, Katerina Angelopoulou, Doxakis Anestakis, Zoi Gerasimina Tampouratzi, Stavros Iliadis, Nikolaos Antoniadis, Alexandros Giakoustidis, Apostolos Papalois, Vasileios Papadopoulos, Theofilos Poutahidis, Dimitrios Giakoustidis
Rok vydání: 2023
Předmět:
Zdroj: Journal of Clinical Medicine
Volume 12
Issue 5
Pages: 1751
ISSN: 2077-0383
Popis: Neutrophil and T-cell recruitment contribute to hepatic ischemia/reperfusion injury. The initial inflammatory response is orchestrated by Kupffer cells and liver sinusoid endothelial cells. However, other cell types, including γδ-Τ cells, seem to be key mediators in further inflammatory cell recruitment and proinflammatory cytokine release, including IL17a. In this study, we used an in vivo model of partial hepatic ischemia/reperfusion injury (IRI) to investigate the role of the γδ-Τ-cell receptor (γδTcR) and the role of IL17a in the pathogenesis of liver injury. Forty C57BL6 mice were subjected to 60 min of ischemia followed by 6 h of reperfusion (RN 6339/2/2016). Pretreatment with either anti-γδΤcR antibodies or anti-IL17a antibodies resulted in a reduction in histological and biochemical markers of liver injury as well as neutrophil and T-cell infiltration, inflammatory cytokine production and the downregulation of c-Jun and NF-κΒ. Overall, neutralizing either γδTcR or IL17a seems to have a protective role in liver IRI.
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje