Changes in the seminal plasma proteome of adolescents before and after varicocelectomy
| Autor: | Fabio C. Gozzo, Barbara Ferreira da Silva, Deborah Montagnini Spaine, Agnaldo Pereira Cedenho, Paula Toni Del Giudice, Luiz Fernando Arruda Santos, Eduardo Jorge Pilau, Renato Fraietta, Edson Guimarães Lo Turco, Ricardo Pimenta Bertolla |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Adolescent Proteome Protein digestion Seminal Plasma Proteins Varicocele Semen Biology Male infertility Young Adult Tandem Mass Spectrometry Internal medicine medicine Humans Electrophoresis Gel Two-Dimensional Postoperative Period Albumin Obstetrics and Gynecology medicine.disease Blood proteins Urogenital Surgical Procedures Endocrinology Reproductive Medicine Prostatic acid phosphatase Preoperative Period |
| Zdroj: | Fertility and sterility. 100(3) |
| ISSN: | 1556-5653 |
| Popis: | Objective To compare seminal plasma protein profiles before and after varicocele correction to assess if surgical intervention alters the protein profile. Design Prospective study. Setting Academic research environment. Patient(s) Nineteen adolescent boys with varicocele grades II or III. Intervention(s) Two semen samples were collected before bilateral subinguinal microsurgical varicocelectomy, and two semen samples were collected 3 months after surgery. Seminal plasma protein profiles were determined with the use of two-dimensional gel electrophoresis. Proteins were separated in 18-cm 3–10 pH strips and 10%–17.5% gradient gels. Gels were stained, scanned, and compared with the use of Imagemaster 2D platinum 7.0. Spots of interest were removed from gels, and protein digestion was performed with the use of trypsin. Digests were identified with the use of electrospray ionization-quadrupole/time-of-flight tandem mass spectrometry (ESI-QTOF MS/MS), and spectra were analyzed with the use of the Mascot software. Main Outcome Measure(s) Proteins uniquely or overexpressed in each period (before or after varicocelectomy). Result(s) Nineteen spots were differentially expressed between pre- and postsurgery samples. Identified proteins were albumin, proteasome subunit alpha type 6, alpha-1-antitrypsin, fibronectin, CD177, prostatic acid phosphatase, specific prostatic antigen, alpha-2-antiplasmin, vitamin D–binding protein, gastricsin, clusterin, semenogelin-1, semenogelin-2, superoxide dismutase, protein–glutamine gamma glutamyltransferase-4, and prolactin-inducing protein. Conclusion(s) Varicocelectomy is associated with changes in the seminal plasma protein profile. Understanding specific pathways leading to male infertility may further assist physicians in demonstrating deviation from homeostasis in male infertility. In addition, it may be possible to observe if surgical intervention does indeed revert altered pathways toward a homeostatic state. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|