Genetic typing of the mouse ob mutation by PCR and restriction enzyme analysis
| Autor: | Susumu Makino, Tadao Ohara, Tsutomu Hirasawa |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Genotype
Restriction enzyme analysis Mice Obese Biology medicine.disease_cause Polymerase Chain Reaction General Biochemistry Genetics and Molecular Biology Mice Biopsy medicine Animals Obesity Genotyping Genetics Mutation General Veterinary medicine.diagnostic_test Heterozygote advantage General Medicine DNA Restriction Enzymes Molecular biology Mice Inbred C57BL Genetic typing Animal Science and Zoology Restriction fragment length polymorphism Polymorphism Restriction Fragment Length |
| Zdroj: | Experimental animals. 46(1) |
| ISSN: | 1341-1357 |
| Popis: | A genetic typing method for the mouse obese (ob) mutation by PCR and restriction fragment length polymorphism (RFLP) analysis was developed. Three genotypes (ob/ob, ob/+ and +/+) of the ob mouse are rapidly differentiated with this assay. Since only a small biopsy specimen (tail end) is necessary for the genotyping, the ob mouse at any age is typable and can be used in subsequent breeding or research. Obese homozygotes (ob/ob) were efficiently produced by mating heterozygotes (ob/+) only, which were selected by using the PCR-RFLP assay. |
| Databáze: | OpenAIRE |
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