A genome-wide 3C-method for characterizing the three-dimensional architectures of genomes
| Autor: | William Stafford Noble, Stanley Fields, Kevin Schutz, Jay Shendure, Zhijun Duan, Choli Lee, Mirela Andronescu, C. Anthony Blau |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
DNA repair
Locus (genetics) Computational biology Saccharomyces cerevisiae Biology Genome General Biochemistry Genetics and Molecular Biology Article Chromosome conformation capture Formaldehyde Animals Humans Genomic library Biotinylation DNA Cleavage DNA Fungal Molecular Biology Gene Library Genetics Fungal genetics Chromosome Chromosome Mapping DNA Restriction Enzymes Sequence Analysis DNA Cross-Linking Reagents ROC Curve DECIPHER Nucleic Acid Conformation DNA Circular Genome Fungal |
| Popis: | Accumulating evidence demonstrates that the three-dimensional (3D) organization of chromosomes within the eukaryotic nucleus reflects and influences genomic activities, including transcription, DNA replication, recombination and DNA repair. In order to uncover structure-function relationships, it is necessary first to understand the principles underlying the folding and the 3D arrangement of chromosomes. Chromosome conformation capture (3C) provides a powerful tool for detecting interactions within and between chromosomes. A high throughput derivative of 3C, chromosome conformation capture on chip (4C), executes a genome-wide interrogation of interaction partners for a given locus. We recently developed a new method, a derivative of 3C and 4C, which, similar to Hi-C, is capable of comprehensively identifying long-range chromosome interactions throughout a genome in an unbiased fashion. Hence, our method can be applied to decipher the 3D architectures of genomes. Here, we provide a detailed protocol for this method. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|