TRPA1 Is Functionally Expressed Primarily by IB4-Binding, Non-Peptidergic Mouse and Rat Sensory Neurons
| Autor: | Cheryl L. Stucky, Elena A. Kossyreva, Marie E. Barabas |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Male
Pathology Mouse lcsh:Medicine Somatosensory system Ion Channels Transient receptor potential channel Mice 0302 clinical medicine Transient Receptor Potential Channels Isothiocyanates Ganglia Spinal Acrolein lcsh:Science Receptor TRPA1 Cation Channel Mice Knockout Neurons 0303 health sciences Multidisciplinary food and beverages Animal Models Exons Calcium Imaging Cell biology Female Plant Lectins Fura-2 psychological phenomena and processes Research Article Protein Binding Agonist medicine.medical_specialty medicine.drug_class Green Fluorescent Proteins Neuropeptide Neurophysiology Neuroimaging Biology Calcitonin gene-related peptide 03 medical and health sciences Calcium imaging Model Organisms Peripheral Nervous System medicine Animals 030304 developmental biology TRPC Cation Channels Gene Expression Profiling lcsh:R Rats Mice Inbred C57BL Nerve growth factor nervous system Gene Expression Regulation Cellular Neuroscience Rat lcsh:Q Molecular Neuroscience 030217 neurology & neurosurgery Neuroscience |
| Zdroj: | PLoS ONE PLoS ONE, Vol 7, Iss 10, p e47988 (2012) |
| ISSN: | 1932-6203 |
| Popis: | Subpopulations of somatosensory neurons are characterized by functional properties and expression of receptor proteins and surface markers. CGRP expression and IB4-binding are commonly used to define peptidergic and non-peptidergic subpopulations. TRPA1 is a polymodal, plasma membrane ion channel that contributes to mechanical and cold hypersensitivity during tissue injury, making it a key target for pain therapeutics. Some studies have shown that TRPA1 is predominantly expressed by peptidergic sensory neurons, but others indicate that TRPA1 is expressed extensively within non-peptidergic, IB4-binding neurons. We used FURA-2 calcium imaging to define the functional distribution of TRPA1 among peptidergic and non-peptidergic adult mouse (C57BL/6J) DRG neurons. Approximately 80% of all small-diameter ( |
| Databáze: | OpenAIRE |
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