Role of POMC and AgRP neuronal activities on glycaemia in mice
Autor: | Paula G.F. Quaresma, Young-Bum Kim, Michelle Chung, Aykut Göktürk Üner, Onur Keçik, Wenjing Li, Thiago M. de Araújo, Christian Bjørbæk, Hyon Lee, Hyun Jeong Kim |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Blood Glucose Leptin medicine.medical_specialty lcsh:Medicine Stimulation Biology Carbohydrate metabolism Models Biological Article Diabetes Mellitus Experimental 03 medical and health sciences Eating Mice 0302 clinical medicine Insulin resistance Internal medicine Diabetes mellitus Glucose Intolerance medicine Animals Agouti-Related Protein Receptor lcsh:Science Neurons Multidisciplinary Diabetes digestive oral and skin physiology lcsh:R Insulin sensitivity medicine.disease 3. Good health 030104 developmental biology Endocrinology Glucose nervous system Hypothalamus Feeding behaviour lcsh:Q Proprotein Convertases Insulin Resistance 030217 neurology & neurosurgery hormones hormone substitutes and hormone antagonists |
Zdroj: | Scientific Reports, Vol 9, Iss 1, Pp 1-14 (2019) Scientific Reports |
ISSN: | 2045-2322 |
Popis: | Leptin regulates both feeding and glycaemia primarily through its receptors expressed on agouti-related peptide (AgRP) and pro-opiomelanocortin-expressing (POMC) neurons; however, it is unknown whether activity of these neuronal populations mediates the regulation of these processes. To determine this, we injected Cre-dependent designer receptors exclusively activated by designer drugs (DREADD) viruses into the hypothalamus of normoglycaemic and diabetic AgRP-ires-cre and POMC-cre mice to chemogenetically activate or inhibit these neuronal populations. Despite robust changes in food intake, activation or inhibition of AgRP neurons did not affect glycaemia, while activation caused significant (P = 0.014) impairment in insulin sensitivity. Stimulation of AgRP neurons in diabetic mice reversed leptin’s ability to inhibit feeding but did not counter leptin’s ability to lower blood glucose levels. Notably, the inhibition of POMC neurons stimulated feeding while decreasing glucose levels in normoglycaemic mice. The findings suggest that leptin’s effects on feeding by AgRP neurons are mediated by changes in neuronal firing, while the control of glucose balance by these cells is independent of chemogenetic activation or inhibition. The firing-dependent glucose lowering mechanism within POMC neurons is a potential target for the development of novel anti-diabetic medicines. |
Databáze: | OpenAIRE |
Externí odkaz: | |
Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |