Phenotypical and functional characteristics of mesenchymal stem cells from bone marrow: comparison of culture using different media supplemented with human platelet lysate or fetal bovine serum
| Autor: | Elfi Ducrocq, Nesrine Ben Azouna, Tarek Ben Othman, Jorge Domenech, F. Jenhani, Lamia Berraeis, Zohra Regaya |
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| Rok vydání: | 2011 |
| Předmět: |
Blood Platelets
Cell Extracts medicine.medical_treatment Cellular differentiation Medicine (miscellaneous) Bone Marrow Cells Biology Biochemistry Genetics and Molecular Biology (miscellaneous) Andrology medicine Animals Humans Cells Cultured Stem cell transplantation for articular cartilage repair Interleukin-6 Research Mesenchymal stem cell Interleukin-8 Cell Differentiation Mesenchymal Stem Cells Cell Biology Culture Media Cytokine medicine.anatomical_structure Phenotype Immunology Molecular Medicine Receptors Parathyroid Hormone Cytokine secretion Cattle Bone marrow Stem cell Receptors Calcium-Sensing Fetal bovine serum |
| Zdroj: | Stem Cell Research & Therapy |
| ISSN: | 1757-6512 |
| Popis: | Introduction Mesenchymal stem cells (MSCs) are multipotent cells able to differentiate into several mesenchymal lineages, classically derived from bone marrow (BM) but potentially from umbilical cord blood (UCB). Although they are becoming a good tool for regenerative medicine, they usually need to be expanded in fetal bovine serum (FBS)-supplemented media. Human platelet lysate (HPL) has recently been proposed as substitute for safety reasons, but it is not yet clear how this supplement influences the properties of expanded MSCs. Methods In the present study, we compared the effect of various media combining autologous HPL with or without FBS on phenotypic, proliferative and functional (differentiation, cytokine secretion profile) characteristics of human BM-derived MSCs. Results Despite less expression of adipogenic and osteogenic markers, MSCs cultured in HPL-supplemented media fully differentiated along osteoblastic, adipogenic, chondrogenic and vascular smooth muscle lineages. The analyses of particular specific proteins expressed during osteogenic differentiation (calcium-sensing receptor (CaSR) and parathormone receptor (PTHR)) showed their decrease at D0 before any induction for MSC cultured with HPL mostly at high percentage (10%HPL). The cytokine dosage showed a clear increase of proliferation capacity and interleukin (IL)-6 and IL-8 secretion. Conclusions This study shows that MSCs can be expanded in media supplemented with HPL that can totally replace FBS. HPL-supplemented media not only preserves their phenotype as well as their differentiation capacity, but also shortens culture time by increasing their growth rate. |
| Databáze: | OpenAIRE |
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