Anti-Inflammatory Activities of Captopril and Diuretics on Macrophage Activity in Mouse Humoral Immune Response
| Autor: | Janusz Marcinkiewicz, Paweł Bryniarski, Katarzyna Nazimek |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Captopril QH301-705.5 Phagocytosis Anti-Inflammatory Agents Pharmacology Article Catalysis Inorganic Chemistry Mice Immune system Antigen humoral immunity medicine Macrophage Animals Physical and Theoretical Chemistry hypotensive drugs Biology (General) Molecular Biology antihypertensive therapy QD1-999 Spectroscopy Antihypertensive Agents Sheep biology business.industry Organic Chemistry Furosemide General Medicine diuretics Computer Science Applications Immunity Humoral macrophages Chemistry Humoral immunity biology.protein Drug Therapy Combination Antibody business medicine.drug |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 11374, p 11374 (2021) International Journal of Molecular Sciences Volume 22 Issue 21 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | Hypertension is accompanied by the over-activation of macrophages. Diuretics administered alone or in combination with hypotensive drugs may have immunomodulatory effects. Thus, the influence of tested drugs on mouse macrophage-mediated humoral immunity was investigated. Mice were treated intraperitoneally with captopril (5 mg/kg) with or without hydrochlorothiazide (10 mg/kg) or furosemide (5 mg/kg) by 8 days. Mineral oil-induced peritoneal macrophages were harvested to assess the generation of cytokines in ELISA, and the expression of surface markers was analyzed cytometrically. Macrophages were also pulsed with sheep red blood cells (SRBC) and transferred to naive mice for evaluation of their ability to induce a humoral immune response. Tested drugs increase the expression of surface markers important for the antigen phagocytosis and presentation. SRBC-pulsed macrophages from mice treated with captopril combined with diuretics increased the secretion of antigen-specific antibodies by recipient B cells, while macrophages of mice treated with hydrochlorothiazide or furosemide with captopril increased the number of antigen-specific B cells. Tested drugs alter the macrophage secretory profile in favor of anti-inflammatory cytokines. Our results showed that diuretics with or without captopril modulate the humoral response by affecting the function of macrophages, which has significant translational potential in assessing the safety of antihypertensive therapy. |
| Databáze: | OpenAIRE |
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