Hymenobacter guriensis sp. nov., and Hymenobacter duratus sp. nov., Radiation-Resistant Species Isolated from Soil in South Korea
| Autor: | Myung Kyum Kim, Tuvshinzaya Damdintogtokh, In-Tae Cha |
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| Rok vydání: | 2021 |
| Předmět: |
DNA
Bacterial food.ingredient Sequence analysis Cytophagaceae Biology Tibet medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Hymenobacter perfusus Soil food RNA Ribosomal 16S Hymenobacter kanuolensis Hymenobacter Republic of Korea Hymenobacter tibetensis medicine Phylogeny Soil Microbiology Base Composition Hymenobacter psychrotolerans Strain (chemistry) Bacteroidetes Fatty Acids Sequence Analysis DNA General Medicine 16S ribosomal RNA Bacterial Typing Techniques |
| Zdroj: | Current Microbiology. 78:3334-3341 |
| ISSN: | 1432-0991 0343-8651 |
| DOI: | 10.1007/s00284-021-02517-6 |
| Popis: | Two novel Gram-stain-negative, non-motile, aerobic, rod-shaped, circular, convex, red-colored and UV-tolerant strains BT594T and BT646T were isolated from soil collected in Guri city (37° 36' 0″ N, 127° 9' 0″ E) and Gwangju city (37° 22' 0″ N, 127° 17' 0″ E), respectively, South Korea. 16S rDNA sequence analysis indicated that strains BT594T and BT646T belong to a distinct lineage within the genus Hymenobacter (family Hymenobacteraceae, order Cytophagales, class Cytophagia, phylum Bacteroidetes, kingdom Bacteria). The 16S rDNA gene sequence similarity between the two strains BT594T and BT646T was 96.2%. The strain BT594T was closely related to Hymenobacter psychrotolerans Tibet-IIU11T (97.0% 16S rDNA gene similarity) and Hymenobacter tibetensis XTM003T (96.3%). The strain BT646T was closely related to Hymenobacter psychrotolerans Tibet-IIU11T (98.6%), Hymenobacter kanuolensis T-3 T (96.8%) and Hymenobacter perfusus LMG 26000 T (96.7%). The two strains were found to have the same quinone system, with MK-7 as the major respiratory quinone. The major polar lipids of strains BT594T and BT646T were phosphatidylethanolamine (PE) and aminophospholipids (APL). The major cellular fatty acids of strain BT594T were anteiso-C15:0 (17.9%), iso-C15:0 (16.1%) and summed feature 3 (C16:1 ω6c / C16:1 ω7c) (10.0%). The major cellular fatty acids of strain BT646T were summed feature 3 (C16:1 ω6c / C16:1 ω7c) (18.3%), C16:0 (17.2%) and summed feature 4 (iso-C17:1 I / anteiso-C17:1 B) (14.5%). Based on the polyphasic analysis, strains BT594T and BT646T can be suggested as two novel bacterial species within the genus Hymenobacter and the proposed names are Hymenobacter guriensis and Hymenobacter duratus, respectively. The type strain of Hymenobacter guriensis is BT594T (= KCTC 21863 T = NBRC 114853 T) and the type strain of Hymenobacter duratus is BT646T (= KCTC 21915 T = NBRC 114854 T). |
| Databáze: | OpenAIRE |
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