Biochemical and molecular diagnosis of insecticide resistance conferred by esterase, MACE, kdr and super-kdr based mechanisms in Italian strains of the peach potato aphid, Myzus persicae (Sulzer)
| Autor: | Piero Cravedi, Emanuele Mazzoni, Davide Bizzaro, Angela Criniti, Gian Carlo Manicardi, Alessandro Tondelli, Stefano Cassanelli |
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| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
RFLP-PCR
Linkage disequilibrium Pesticide resistance Health Toxicology and Mutagenesis Myzus persicae MACE AChE Esterase pyrethroids parasitic diseases Genotype biochemical and-molecular diagnosis Bioassay kdr esterase Genetics biology screening insecticide resistance super-kdr Aphididae General Medicine biology.organism_classification Settore AGR/11 - ENTOMOLOGIA GENERALE E APPLICATA kdr resistance Restriction fragment length polymorphism Agronomy and Crop Science sodium channel |
| Popis: | In this paper we analysed the basis of insecticide resistance in 59 Italian strains of the peach potato aphid Myzus persicae using both molecular and biochemical assays. Our data as a whole clearly indicate that most M. persicae strains (76.3%) have high or extremely high production of an esterase enzyme which sequester and detoxify insecticides with esteric group. Kdr genotypes conferring resistance towards pyrethoids are present in 57.7% of the analysed populations. Moreover, 26.5% of the kdr positive strains possess also the M918T mutation conferring super- kdr phenotype. Strains with modified AChE (MACE) are not so numerous (27.1%), although they can be found almost everywhere in Italy. Considering all the strains analysed, both MACE and kdr phenotypes are associated with high levels of esterase activity. In Central–Southern regions, kdr and MACE resistance mechanisms resulted in linkage disequilibrium. Bioassays performed in order to evaluate the efficacy of a pyrethroid insecticide against a strain possessing a F979S mutation within its para-type sodium channel gene suggests that this amino acid substitution could affect the sodium channel responsivity to pyrethroids. |
| Databáze: | OpenAIRE |
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