A reference microsatellite kit to assess for genetic diversity of Sorghum bicolor (Poaceae)
| Autor: | Jean-Louis Noyer, Punna Ramu, Jean-François Rami, Claire Billot, Patrick Wincker, Ronan Rivallan, Ange-Marie Risterucci, Charles Tom Hash, Mbaye Ndoye Sall, Jean-Christophe Glaszmann, Monique Deu, Daniel Fonceka |
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| Rok vydání: | 2012 |
| Předmět: |
Genotyping Techniques
Sélection Plant Science Polymerase Chain Reaction Genetic analysis F30 - Génétique et amélioration des plantes Genotype Marqueur génétique Genetics biology Reproduction food and beverages Sorghum bicolor Microsatellite Génotype DNA Plant F60 - Physiologie et biochimie végétale Molecular Sequence Data Polymorphisme Variation génétique Species Specificity Genotyping Alleles Sorghum Ecology Evolution Behavior and Systematics DNA Primers Genetic diversity Polymorphism Genetic business.industry Genetic Variation Sequence Analysis DNA biology.organism_classification Biotechnology Genetic marker business Sweet sorghum Microsatellite Repeats |
| Zdroj: | American Journal of Botany |
| ISSN: | 1537-2197 0002-9122 |
| Popis: | Premise of the study: Discrepancies in terms of genotyping data are frequently observed when comparing simple sequence repeat (SSR) data sets across genotyping technologies and laboratories. This technical concern introduces biases that hamper any synthetic studies or comparison of genetic diversity between collections. To prevent this for Sorghum bicolor , we developed a control kit of 48 SSR markers. Methods and Results: One hundred seventeen markers were selected along the genome to provide coverage across the length of all 10 sorghum linkage groups. They were tested for polymorphism and reproducibility across two laboratories (Centre de Cooperation Internationale en Recherche Agronomique pour le Developpement [CIRAD], France, and International Crops Research Institute for the Semi-Arid Tropics [ICRISAT], India) using two commonly used genotyping technologies (polyacrylamide gel-based technology with LI-COR sequencing machines and capillary systems with ABI sequencing apparatus) with DNA samples from a diverse set of 48 S. bicolor accessions. Conclusions: A kit for diversity analysis (http://sat.cirad.fr/sat/sorghum_SSR_kit/) was developed. It contains information on 48 technically robust sorghum microsatellite markers and 10 DNA controls. It can further be used to calibrate sorghum SSR genotyping data acquired with different technologies and compare those to genetic diversity references. (Resume d'auteur) |
| Databáze: | OpenAIRE |
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