Metabolic profiling of strawberry grape ( Vitis × labruscana cv. 'Isabella') components by nuclear magnetic resonance (NMR) and evaluation of their antioxidant and antiproliferative properties
Autor: | Simona Piccolella, Nicoletta Potenza, Marialuisa Gallicchio, Monica Scognamiglio, Brigida D'Abrosca, Antonio Fiorentino, Aniello Russo, Pietro Monaco, Severina Pacifico |
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Přispěvatelé: | Pacifico, Severina, D'Abrosca, Brigida, Scognamiglio, M, Gallicchio, M, Potenza, Nicoletta, Piccolella, Simona, Russo, Aniello, Monaco, Pietro, Fiorentino, Antonio |
Rok vydání: | 2011 |
Předmět: |
antiproliferative activity
Antioxidant Magnetic Resonance Spectroscopy DPPH medicine.medical_treatment Flavonoid radical scavenging capacity antioxidant activity Antioxidants Cell Line chemistry.chemical_compound medicine Caffeic acid Humans Vitis Food science Cell Proliferation chemistry.chemical_classification ABTS Plant Extracts metabolic profiling food and beverages NMR analysi General Chemistry Vitis × labruscana chemistry Biochemistry Stalk 'Isabella' grape Propionate Metabolome General Agricultural and Biological Sciences Quercetin |
Zdroj: | Journal of agricultural and food chemistry. 59(14) |
ISSN: | 1520-5118 |
Popis: | In the assessment of the antioxidant properties of edible plants, the widely consumed Vitis × labruscana cv. 'Isabella', known in Italy as "fragola" (strawberry) grape, was of interest. Phenol and flavonoid contents of the methanolic extracts of peel, pulp, seed, leaf, and stalk components of the plant were determined. The metabolic profile of the extracts was performed by 1D and 2D NMR. Quantitative analysis, obtained in the presence of 0.01% of internal standard trimethylsilyl propionate, evidenced the presence of catechins in both stalk and seed extracts, whereas caffeic acid and quercetin were the main metabolites of the leaf extract. Furthermore, the extracts were tested for their radical scavenging and reducing capacities by measuring their capacity to scavenge DPPH• and ABTS•+and to reduce Fe(III) and Mo(VI) salts. The antioxidant efficacy of the extracts in cell-free systems and their antiproliferative activity toward HepG2 and A549 cells were also evaluated. Seed and stalk components are able to reduce by 39.6 and 40.6%, respectively, the amount of the metabolically active HepG2 cells after only 24 h of exposure. © 2011 American Chemical Society. |
Databáze: | OpenAIRE |
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