Combining Catalyst-Free Click Chemistry with Coaxial Electrospinning to Obtain Long-Term, Water-Stable, Bioactive Elastin-Like Fibers for Tissue Engineering Applications

Autor: Alicia Fernández-Colino, Stephan Rütten, Stefan Jockenhoevel, Petra Mela, Frederic Wolf, José Carlos Rodríguez-Cabello
Přispěvatelé: AMIBM, RS: FSE AMIBM, Sciences, RS: FSE Sciences, Biobased Materials, RS: FSE Biobased Materials
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
RECOMBINAMERS
Materials science
Polymers and Plastics
STRATEGIES
BIOCONJUGATION
Myocytes
Smooth Muscle
FABRICATION
Bioengineering
Nanotechnology
02 engineering and technology
ADHESION
coaxial electrospinning
Biomaterials
03 medical and health sciences
Tissue engineering
elastin-like fibers
DESIGN
Materials Chemistry
Humans
Cells
Cultured
chemistry.chemical_classification
Bioprosthesis
Aqueous solution
Bioconjugation
biology
Tissue Engineering
Tissue Scaffolds
cardiovascular
DIAMETER
PROLIFERATION
Endothelial Cells
NANOFIBROUS SCAFFOLDS
Polymer
Adhesion
021001 nanoscience & nanotechnology
Electrospinning
Blood Vessel Prosthesis
Elastin
one-step processes
030104 developmental biology
chemistry
click chemistry
biology.protein
Click chemistry
0210 nano-technology
MATRICES
Biotechnology
Zdroj: Macromolecular Bioscience, 18(11):1800147. John Wiley & Sons Inc.
ISSN: 1616-5187
DOI: 10.1002/mabi.201800147
Popis: Elastic fibers are a fundamental requirement for tissue-engineered equivalents of physiologically elastic native tissues. Here, a simple one-step electrospinning approach is developed, combining i) catalyst-free click chemistry, ii) coaxial electrospinning, and iii) recombinant elastin-like polymers as a relevant class of biomaterials. Water-stable elastin-like fibers are obtained without the use of cross-linking agents, catalysts, or harmful organic solvents. The fibers can be directly exposed to an aqueous environment at physiological temperature and their morphology maintained for at least 3 months. The bioactivity of the fibers is demonstrated with human vascular cells and the potential of the process for vascular tissue engineering is shown by fabricating small-diameter tubular fibrous scaffolds. Moreover, highly porous fluffy 3D constructs are obtained without the use of specially designed collectors or sacrificial materials, further supporting their applicability in the biomedical field. Ultimately, the strategy that is developed here may be applied to other click systems, contributing to expanding their potential in medical technology.
Databáze: OpenAIRE
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