ISOLATES OF SARCOCYSTIS FALCATULA–LIKE ORGANISMS FROM SOUTH AMERICAN OPOSSUMS DIDELPHIS MARSUPIALIS AND DIDELPHIS ALBIVENTRIS FROM SÃO PAULO, BRAZIL
| Autor: | C. E. Kerber, David S. Lindsay, Benjamin M. Rosenthal, Solange Maria Gennari, Nobuko Kasai, Jitender P. Dubey, S. K. Shen, Oliver C.H. Kwok, H. F. J. Pena |
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| Rok vydání: | 2001 |
| Předmět: |
biology
Didelphis Molecular Sequence Data Sarcocystis Zoology Opossums DNA Protozoan Melopsittacus biology.organism_classification Polymerase Chain Reaction Didelphis albiventris Parasitology Budgerigar biology.animal parasitic diseases Animals Protozoa Parasite hosting Brazil Cells Cultured Ecology Evolution Behavior and Systematics |
| Zdroj: | Journal of Parasitology. 87:1449-1453 |
| ISSN: | 1937-2345 0022-3395 |
| Popis: | Isolates of Sarcocystis falcatula-like organisms from South American opossums were characterized based on biological and morphological criteria. Sporocysts from intestinal scrapings of 1 Didelphis marsupialis and 8 Didelphis albiventris from São Paulo, Brazil, were fed to captive budgerigars (Melopsittacus undulatus). Budgerigars fed sporocysts from all 9 isolates became ill and S. falcatula-like schizonts were identified in sections of their lungs by immunohistochemical staining. Sarcocystis falcatula-like organisms were cultured from lungs of budgerigars fed sporocysts from D. marsupialis and from lungs of budgerigars fed sporocysts from 3 of 8 D. albiventris. The 33/54 locus amplified by polymerase chain reaction from culture-derived merozoites contained both a HinfI endonuclease recognition site previously suggested to diagnose S. falcatula and a DraI site thought to diagnosed S. neurona. Development of the isolate from D. marsupialis was studied in cell culture; its schizonts divided by endopolygeny, leaving a residual body. Morphological and genetic variation differentiated this Sarcocystis isolate originating in D. marsupialis from the Cornell I isolate of S. falcatula. This is the first report of a S. falcatula infection in the South American opossum, D. marsupialis. |
| Databáze: | OpenAIRE |
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