Morphology, kinetics and secretory activity of antibody-forming cells
| Autor: | M.V.D. Ende, N Kors, A.A. Geldof, H. L. Langevoort, P. Rijnhart |
|---|---|
| Přispěvatelé: | Urology, CCA - Cancer biology and immunology, CCA - Imaging and biomarkers |
| Rok vydání: | 1984 |
| Předmět: |
Pathology
medicine.medical_specialty Immunology Cell Population Plasma Cells Priming (immunology) Spleen Bone Marrow Cells Plasma cell Horseradish peroxidase Immunoenzyme Techniques Mice Bone Marrow medicine Immunology and Allergy Animals education Antibody-Producing Cells Horseradish Peroxidase education.field_of_study biology Hematology Molecular biology Kinetics medicine.anatomical_structure biology.protein Mice Inbred CBA Autoradiography Female Immunization Bone marrow Lymph Nodes Antibody Cell Division |
| Zdroj: | Immunobiology, 166(3), 296-307. Urban und Fischer Verlag GmbH und Co. KG Geldof, A A, Rijnhart, P, Ende, M V D, Kors, N & Langevoort, H L 1984, ' Morphology, Kinetics and Secretory Activity of Antibody-forming Cells ', Immunobiology, vol. 166, no. 3, pp. 296-307 . https://doi.org/10.1016/S0171-2985(84)80047-9 |
| ISSN: | 0171-2985 |
| Popis: | Specific antibody-forming cells from spleen, bone marrow and popliteal lymph nodes were studied in mice after subcutaneous priming and intravenous boosting with horseradish peroxidase (HRP). Functional antibody-secreting capacity of these cells was correlated with their morphology at the cell population level. For this purpose, cells synthesizing anti-HRP antibody from the same cell suspensions were studied simultaneously by light and electron microscopy and by a plaque assay. It appeared that the population of cells responsible for antibody synthesis as well as antibody secretion was morphologically heterogeneous: besides plasma cells, considerable numbers of antibody-forming lymphocytes, antibody-forming plasmablasts and antibody-forming immature plasma cells were observed. Immature plasma cells constituted the majority of antibody-forming as well as antibody-secreting cells. Among the immature plasma cells in the popliteal lymph nodes proliferation occurred. Evidence is presented that the light-microscopically identified mature plasma cell is not the main antibody-forming cell. It does not show 3H-Thymidine incorporation and should be considered as a non-dividing end-cell. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|