Pro- and anti-inflammatory responses of common carp Cyprinus carpio head kidney leukocytes to E.coli LPS as modified by different dietary plant oils

Autor: Thi Mai Nguyen, Syaghalirwa N.M. Mandiki, Thu Hang Nguyen, Nang Thu Tran Thi, Jean M.A.J. Salomon, Patrick Kestemont, Truong Quynh Nhu, Joel Bondekwe Baruti
Rok vydání: 2020
Předmět:
Fish Proteins
Lipopolysaccharides
food.ingredient
Carps
LC-PUFAs
Immunology
Biology
NO activity
Immunomodulation
03 medical and health sciences
chemistry.chemical_compound
Common carp
food
Linseed oil
Escherichia coli
Leukocytes
Animals
Plant Oils
Food science
Cytokine
Cells
Cultured
Escherichia coli Infections
Peroxidase
030304 developmental biology
chemistry.chemical_classification
Inflammation
Total Ig
0303 health sciences
Eicosanoid metabolism
food and beverages
04 agricultural and veterinary sciences
Cod liver oil
Eicosanoid
Fish oil
Head Kidney
Lipid Metabolism
Diet
chemistry
Docosahexaenoic acid
Cytoprotection
040102 fisheries
Fatty Acids
Unsaturated
0401 agriculture
forestry
and fisheries
Cytokines
lipids (amino acids
peptides
and proteins)
Arachidonic acid
Inflammation Mediators
Developmental Biology
Polyunsaturated fatty acid
Zdroj: Developmental and comparative immunology. 114
ISSN: 1879-0089
Popis: Dietary lipids could modify fatty acid (FA) composition in fish tissues. Long chain polyunsaturated fatty acids (LC-PUFAs) such as arachidonic acid (ARA), eicosapentaneoic acid (EPA) and docosahexaenoic acid (DHA) are able to modulate the immune status in fish through an inflammatory process but their availability may be limited when fish are exclusively fed plant oils. This study was conducted to evaluate how to maximise the utilisation of dietary plant oil for an efficient inflammatory response in common carp head kidney leukocytes (HKLs) exposed to a gram-negative bacterial endotoxin, Escherichia coli lipopolysaccharides (LPS). HKLs were isolated from fish fed cod liver oil (CLO), linseed oil (LO), sesame oil (SO) a blend of SO and LO (SLO, v:v 1:1), and these plant oil diets supplemented with DHA (SO + DHA, SOD) or ARA (LO + ARA, LOA) for 6 weeks. Cells were then exposed to LPS at a dose of 10 μg/mL for 4 and 24 h. Peroxidase activity, total Ig, and NO levels were measured in the culture medium, while cells were used for expression analyses of candidate genes in pattern recognition (tlr-4), eicosanoid metabolism (pge2, 5-lox), pro-inflammatory (il-1, il-6, il-8, tnf-α, nf-kb, inos, cxc), anti-inflammatory (il-10, nf-kbi, tgf-β1) responses, and cytoprotective (gpx-1, prdx-3) processes. Results showed that LPS induced significantly inflammatory responses, evidenced by a high level of almost all the targeted humoral immune parameters and/or gene expression. Expression of inflammatory cytokines and other inflammatory mediators was upregulated after 4 h-LPS exposure and reverted to basal levels after 24 h. HKLs from fish fed SLO, LOA, or SOD diet exhibited a more efficient regulation of acute inflammatory processes than those fed CLO diet. The results indicate that the immune competence of fish fed plant oil mixture was comparable to the one of fish fed fish oil diet. Moreover, the supplementation of ARA or DHA induced similar immunomodulation in common carp.
Databáze: OpenAIRE
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