Active extracts of black tea (Camellia Sinensis) induce apoptosis of PC-3 prostate cancer cells via mitochondrial dysfunction
| Autor: | Dong Chen, Caijin Ling, Chaoyi Zhao, Aiqing Miao, Jinchi Tang, Shili Sun, Shi Pang, Shunshun Pan |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
Cancer Research Necrosis Cell Survival Cell Apoptosis Mitochondrion Biology Camellia sinensis Catechin Adenosine Triphosphate Cell Line Tumor medicine Biflavonoids Humans Cell Proliferation Membrane Potential Mitochondrial Oncogene Caspase 3 Cell growth Cytochromes c Polyphenols Prostatic Neoplasms General Medicine Cell cycle Molecular biology Mitochondria Cytosol medicine.anatomical_structure Oncology Cancer research medicine.symptom |
| Zdroj: | Oncology Reports. 30:763-772 |
| ISSN: | 1791-2431 1021-335X |
| DOI: | 10.3892/or.2013.2504 |
| Popis: | Cancer of the prostate gland is the most common invasive malignancy and the second leading cause of cancer-related death in human males. Many studies have shown that black tea reduces the risk of several types of cancer. We studied the effects of active extracts of black tea and the black tea polyphenols theaflavins (TFs), on the cellular proliferation and mitochondria of the human prostate cancer cell line PC-3. Our studies revealed that Yinghong black tea extracts (YBT), Assam black tea extracts (ABT) and TFs inhibited cell proliferation in a dose-dependent manner. We also showed that TFs, YBT and ABT affected the morphology of PC-3 cells and induced apoptosis or even necrosis in PC-3 cells. In addition, it was observed that the samples significantly caused loss of the mitochondrial membrane potential, release of cytochrome c from the intermembrane space into the cytosol, decrease of the ATP content and activation of caspase-3 compared with the control. Taken together, these findings suggest that black tea could act as an effective anti-proliferative agent in PC-3 cells, and TFs, YBT and ABT induced apoptosis of PC-3 cells through mitochondrial dysfunction. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|