An important role of phospholipase Cγ1 in pre-B-cell development and allelic exclusion
| Autor: | Guoping Fu, Shoua Yang, Debra K. Newman, Demin Wang, Weiguo Zhang, James Schuman, Renren Wen, Yuhong Chen |
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| Rok vydání: | 2004 |
| Předmět: |
Transgene
Mice Transgenic Phospholipase Phospholipase C gamma Biology Article General Biochemistry Genetics and Molecular Biology Mice medicine Animals Progenitor cell Receptor Molecular Biology Alleles Crosses Genetic B cell Mice Knockout B-Lymphocytes General Immunology and Microbiology General Neuroscience Hematopoietic Stem Cells Molecular biology Allelic exclusion medicine.anatomical_structure Type C Phospholipases Immunoglobulin heavy chain |
| Zdroj: | The EMBO Journal. 23:4007-4017 |
| ISSN: | 1460-2075 0261-4189 |
| DOI: | 10.1038/sj.emboj.7600405 |
| Popis: | Phospholipase Cgamma1 (PLCgamma1) has been reported to be expressed predominantly in T cells and to play an important role in T-cell receptor signaling. Here we show that PLCgamma1 is expressed throughout B-cell development, with high expression in B-cell progenitors, and is involved in pre-B-cell receptor (pre-BCR) signaling. Reduced expression of PLCgamma1, in the absence of PLCgamma2 (PLCgamma1+/-PLCgamma2-/-), impedes early B-cell development at the pro-B- to pre-B-cell transition and impairs immunoglobulin heavy chain allelic exclusion, hallmarks of defective pre-BCR signaling. In contrast, early B-cell development is largely normal, whereas late B-cell maturation is impaired in the absence of PLCgamma2 alone (PLCgamma2-/-) and overexpression of PLCgamma1 in PLCgamma2-/- mice fails to restore BCR-mediated B-cell proliferation and maturation. These studies reveal an essential role of PLCgamma1, distinct from that of PLCgamma2, in B-cell development. |
| Databáze: | OpenAIRE |
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