Transcriptional regulation of Niemann-Pick C1-like 1 gene by liver receptor homolog-1
| Autor: | Eui Sup Lee, Seung H. Han, Su Sun Back, Soo Young Choi, Kyuhyung Han, Hyun Jung Seo, Yeon Ji Jeong, Jin Wook Lee |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
SERBP2
Response element Receptors Cytoplasmic and Nuclear NPC1L1 Biology Cholesterol 7 alpha-hydroxylase Biochemistry Transcriptional regulation Humans Regulatory Elements Transcriptional Liver X receptor Promoter Regions Genetic Molecular Biology Conserved Sequence Cholesterol LRH-1 Transcription Regulation of gene expression Liver receptor homolog-1 Reverse cholesterol transport Membrane Proteins Membrane Transport Proteins General Medicine Hep G2 Cells Molecular biology Gene Expression Regulation Liver Intestinal cholesterol absorption Research Article Sterol Regulatory Element Binding Protein 2 |
| Zdroj: | BMB Reports BMB REPORTS(48): 9 |
| ISSN: | 1976-670X 1976-6696 |
| Popis: | Factors that modulate cholesterol levels have major impacts on cardiovascular disease. Niemann-Pick C1-like 1 (NPC1L1) functions as a sterol transporter mediating intestinal cholesterol absorption and counter-balancing hepatobiliary cholesterol excretion. The liver receptor homolog 1 (LRH-1) had been shown to regulate genes involved in hepatic lipid metabolism and reverse cholesterol transport. To study whether human NPC1L1 gene is regulated transcriptionally by LRH-1, we have analyzed evolutionary conserved regions (ECRs) in HepG2 cells. One ECR was found to be responsive to the LRH-1. Through deletion studies, LRH-1 response element was identified and the binding of LRH-1 was demonstrated by EMSA and ChIP assays. When SREBP2, one of several transcription factors which had been shown to regulate NPC1L1 gene, was co-expressed with LRH-1, synergistic transcriptional activation resulted. In conclusion, we have identified LRH-1 response elements in NPC1L1 gene and propose that LRH-1 and SREBP may play important roles in regulating NPC1L1 gene. [BMB Reports 2015; 48(9): 513-518] |
| Databáze: | OpenAIRE |
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