Different effects of H2O2 treatment on cervical squamous carcinoma cells and adenocarcinoma cells
| Autor: | Haiqin Yin, Nan Peng, Peihai Zhang, Sie Wang, Xiaoyuan Wang, Wenxiang Bi, Yuping Wei |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Pathology
medicine.medical_specialty DTNB hydrogen dioxide cervical neoplasm Superoxide dismutase Andrology chemistry.chemical_compound medicine MTT assay glutathione chemistry.chemical_classification reactive oxygen species Reactive oxygen species biology business.industry Glutathione peroxidase General Medicine Glutathione medicine.disease antioxidative enzyme Squamous carcinoma Basic Research chemistry biology.protein Adenocarcinoma business |
| Zdroj: | Archives of Medical Science : AMS |
| ISSN: | 1896-9151 1734-1922 |
| Popis: | INTRODUCTION This study aims to compare the antioxidant abilities of cervical squamous carcinoma cells and cervical adenocarcinoma cells and to study the related mechanisms. MATERIAL AND METHODS Cervical squamous carcinoma and adenocarcinoma cells were treated with H2O2. Cell proliferation was determined with the MTT assay. The reactive oxygen species (ROS) level was detected by the 2',7'-dichlorofluorescein-diacetate (DCFH-DA) method. The 5,5'-dithiobis-2-nitrobenzoic acid (DTNB) method was performed to measure intracellular concentrations of reduced glutathione (GSH) and oxidized glutathione (GSSG). The nitrite formation method, the molybdate colorimetric method, and the DTNB colorimetric method were used to determine activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), respectively. RESULTS Compared with untreated control cells, cell proliferation of cervical squamous carcinoma cells and cervical adenocarcinoma cells was significantly inhibited by H2O2 treatment (p < 0.05). Reactive oxygen species levels and GSSG levels were significantly increased (p < 0.01), whereas GSH levels were significantly decreased (p < 0.05 or 0.01) in both cells after H2O2 treatment. Thus the ratio of GSH/GSSG was significantly decreased by H2O2 treatment in both cells (p < 0.01). In addition, H2O2 treatment significantly increased activities of SOD, CAT, and GPx in both cells (p < 0.05 or 0.01). Furthermore, the above-mentioned changes induced by H2O2 treatment were more dramatic in cervical squamous carcinoma cells. CONCLUSIONS The antioxidant ability of cervical squamous carcinoma cells is lower than that of cervical adenocarcinoma cells, which may be related to the increased ROS levels in cervical squamous carcinoma cells induced by H2O2 treatments. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|