Differential immuno-kinetic assays of allergen-specific binding for peanut allergy serum analysis
| Autor: | E. R. Kaminski, Rouslan V. Olkhov, Andrew M. Shaw |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Peanut allergy
Immunoglobulin E medicine.disease_cause Biochemistry Analytical Chemistry Mice Dogs Blood serum Allergen Ara h1 Mite medicine Animals Humans Peanut Hypersensitivity Immunoassay biology medicine.diagnostic_test Chemistry food and beverages Allergens Surface Plasmon Resonance biology.organism_classification medicine.disease Kinetics Immunology Cats biology.protein Nanoparticles Gold Antibody Protein Binding |
| Zdroj: | Analytical and Bioanalytical Chemistry. 404:2241-2247 |
| ISSN: | 1618-2650 1618-2642 |
| DOI: | 10.1007/s00216-012-6390-z |
| Popis: | A label-free nanoparticle array platform has been used to detect total peanut allergen-specific binding from whole serum of patients suffering from peanut allergy. The serum from 10 patients was screened against a four-allergen panel of cat and dog dander, dust mite and peanut allergen protein Ara h1. The IgE and IgG contributions to the total specific-binding protein load to Ara h1 were identified using two secondary IgG- and IgE-specific antibodies and were found to contribute less than 50 % of the total specific protein load. The total mass of IgE, IgE and the unresolved specific-binding protein ΔsBP for Ara h1 provides a new serum profile for high-RAST-grade patients 5 and 6 with the IgG/IgE ratio of 4 ± 2 and ΔsBP/IgE ratio of 17 ± 11, neither of which is protective for the small patient cohort. |
| Databáze: | OpenAIRE |
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