Grape seed extract ameliorates bleomycin-induced mouse pulmonary fibrosis
| Autor: | Qi Liu, Ya-nan Liu, Yong-liang Jia, Qiang-min Xie, Xin-wei Dong, Wei Zhao, Jun-xia Jiang, Ling-tian Ge, Yan Guan, Yun Sun |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Pulmonary Fibrosis Anti-Inflammatory Agents Pharmacology Toxicology Bleomycin Proinflammatory cytokine Pulmonary function testing 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Transforming Growth Factor beta Silicosis Fibrosis Pulmonary fibrosis medicine Animals Lung Mice Inbred ICR Antibiotics Antineoplastic Grape Seed Extract business.industry General Medicine medicine.disease Respiratory Function Tests Collagen type I alpha 1 030104 developmental biology medicine.anatomical_structure Matrix Metalloproteinase 9 chemistry 030220 oncology & carcinogenesis Female business Bronchoalveolar Lavage Fluid |
| Zdroj: | Toxicology Letters. 273:1-9 |
| ISSN: | 0378-4274 |
| DOI: | 10.1016/j.toxlet.2017.03.012 |
| Popis: | Pulmonary fibrosis is common in a variety of inflammatory lung diseases, such as interstitial pneumonia, chronic obstructive pulmonary disease, and silicosis. There is currently no effective clinical drug treatment. It has been reported that grape seed extracts (GSE) has extensive pharmacological effects with minimal toxicity. Although it has been found that GSE can improve the lung collagen deposition and fibrosis pathology induced by bleomycin in rat, its effects on pulmonary function, inflammation, growth factors, matrix metalloproteinases and epithelial-mesenchymal transition remain to be researched. In the present study, we studied whether GSE provided protection against bleomycin (BLM)-induced mouse pulmonary fibrosis. ICR strain mice were treated with BLM in order to establish pulmonary fibrosis models. GSE was given daily via intragastric administration for three weeks starting at one day after intratracheal instillation. GSE at 50 or 100mg/kg significantly reduced BLM-induced inflammatory cells infiltration, proinflammatory factor protein expression, and hydroxyproline in lung tissues, and improved pulmonary function in mice. Additionally, treatment with GSE also significantly impaired BLM-induced increases in lung fibrotic marker expression (collagen type I alpha 1 and fibronectin 1) and decreases in an anti-fibrotic marker (E-cadherin). Further investigation indicated that the possible molecular targets of GSE are matrix metalloproteinases-9 (MMP-9) and TGF-β1, given that treatment with GSE significantly prevented BLM-induced increases in MMP-9 and TGF-β1 expression in the lungs. Together, these results suggest that supplementation with GSE may improve the quality of life of lung fibrosis patients by inhibiting MMP-9 and TGF-β1 expression in the lungs. |
| Databáze: | OpenAIRE |
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