The use of apocynin inhibits osteoclastogenesis
| Autor: | Felipe Cordero da Luz, Mariana P. R. Soares, Isadora Akemi Uehara, Marcelo José Barbosa Silva, Ana Paula Lima Oliveira, Paulo Vinicius Gil Alabarse, Erivan Schnaider Ramos, Sandra Y. Fukada, Danielle Pereira Silva, Leda Quercia Vieira |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Osteoclasts Nerve Tissue Proteins Calcium in biology Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Osteogenesis Osteoclast COMPOSTOS ORGÂNICOS medicine Animals chemistry.chemical_classification Reactive oxygen species NADPH oxidase NFATC Transcription Factors biology Voltage-dependent calcium channel Tartrate-Resistant Acid Phosphatase Chemistry Macrophages Acetophenones Membrane Proteins NADPH Oxidases Cell Differentiation Cell Biology General Medicine Molecular biology Acetylcysteine Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Matrix Metalloproteinase 9 RANKL 030220 oncology & carcinogenesis Apocynin biology.protein Female Reactive Oxygen Species Intracellular Signal Transduction |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | Reactive oxygen species (ROS) are produced by NADPH oxidase (NOX), an enzyme that reduces oxygen by using NADPH as a substrate. Apocynin (APO) is a catechol that is used as a NOX inhibitor, and N-acetyl-cysteine (NAC) can reduce intracellular ROS levels. In this work, the effect of APO and NAC on osteoclast formation were evaluated. APO and NAC significantly decreased the number of tartrate-resistant acid phosphatase (TRAP)-positive cells and the osteoclast area. We analyzed bone-marrow derived monocyte-macrophages (BMMs) that differentiated into osteoclasts after RANKL stimulation. Stimulation was associated with either APO or NAC treatment and osteoclastogenesis marker expression, including NFATc1, MMP-9, and DC-STAMP, was evaluated. APO decreased the intracellular calcium concentration by calcium channels other than ITPR1 and TPC2. On the other hand, APO reduced Tnfrsf11a (RANK) expression and did not alter Fam102a (EEIG1) expression. Therefore, our results demonstrate that APO inhibits osteoclastogenesis by the RANK-RANKL-related signaling pathways, decreases osteoclast markers, and reduces intracellular calcium concentration. |
| Databáze: | OpenAIRE |
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