The Biological Role of Hyaluronan-Rich Oocyte-Cumulus Extracellular Matrix in Female Reproduction

Autor: Eva Nagyova
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Review
Extracellular matrix
lcsh:Chemistry
chemistry.chemical_compound
0302 clinical medicine
MG132
Hyaluronic Acid
pentraxin 3
lcsh:QH301-705.5
Spectroscopy
reproductive and urinary physiology
Cumulus Cells
biology
Chemistry
Reproduction
General Medicine
Computer Science Applications
Cell biology
Mifepristone
Serum Amyloid P-Component
medicine.anatomical_structure
C-Reactive Protein
030220 oncology & carcinogenesis
Tumor necrosis factor alpha
Female
hormones
hormone substitutes
and hormone antagonists
oocyte-cumulus complexes
tumor necrosis factor-alpha-induced protein 6
inter-alpha-trypsin inhibitor
endocrine system
Protein family
extracellular matrix
Catalysis
Inorganic Chemistry
hyaluronan
03 medical and health sciences
Progesterone receptor
medicine
Animals
Physical and Theoretical Chemistry
Molecular Biology
Inter-alpha-trypsin inhibitor
urogenital system
Organic Chemistry
Oocyte
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
biology.protein
Oocytes
Cyclooxygenase
Cell Adhesion Molecules
Zdroj: International Journal of Molecular Sciences, Vol 19, Iss 1, p 283 (2018)
International Journal of Molecular Sciences
ISSN: 1422-0067
Popis: Fertilization of the mammalian oocyte requires interactions between spermatozoa and expanded cumulus extracellular matrix (ECM) that surrounds the oocyte. This review focuses on key molecules that play an important role in the formation of the cumulus ECM, generated by the oocyte-cumulus complex. In particular, the specific inhibitors (AG1478, lapatinib, indomethacin and MG132) and progesterone receptor antagonist (RU486) exerting their effects through the remodeling of the ECM of the cumulus cells surrounding the oocyte have been described. After gonadotropin stimulus, cumulus cells expand and form hyaluronan (HA)-rich cumulus ECM. In pigs, the proper structure of the cumulus ECM depends on the interaction between HA and serum-derived proteins of the inter-alpha-trypsin inhibitor (IαI) protein family. We have demonstrated the synthesis of HA by cumulus cells, and the presence of the IαI, tumor necrosis factor-alpha-induced protein 6 and pentraxin 3 in expanding oocyte-cumulus complexes (OCC). We have evaluated the covalent linkage of heavy chains of IαI proteins to HA, as the principal component of the expanded HA-rich cumulus ECM, in porcine OCC cultured in medium with specific inhibitors: AG1478 and lapatinib (both inhibitors of epidermal growth factor receptor tyrosine kinase activity); MG132 (a specific proteasomal inhibitor), indomethacin (cyclooxygenase inhibitor); and progesterone receptor antagonist (RU486). We have found that both RU486 and indomethacin does not disrupt the formation of the covalent linkage between the heavy chains of IαI to HA in the expanded OCC. In contrast, the inhibitors AG1478 and lapatinib prevent gonadotropin-induced cumulus expansion. Finally, the formation of oocyte-cumulus ECM relying on the covalent transfer of heavy chains of IαI molecules to HA has been inhibited in the presence of MG132.
Databáze: OpenAIRE
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